Construction of a trehalose-6-phosphate synthase gene driven by drought-responsive promoter and expression of drought-resistance in transgenic tobacco

被引：0
|
作者：
Zhao, HW [1 ]
Chen, YJ [1 ]
Hu, YL [1 ]
Gao, Y [1 ]
Lin, ZP [1 ]
机构：
[1] Peking Univ, Coll Life Sci, Natl Lab Prot Engn & Plant Genet Engn, Beijing 100871, Peoples R China
来源：
ACTA BOTANICA SINICA | 2000年 / 42卷 / 06期
关键词：
drought-responsive promoter; trehalose-6-phosphate synthase; Nicotiana tabacum; drought tolerance;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The trehalose-6-phosphate synthase gene (TPS) from Saccharomyces cerevisiae Hansen and the drought-responsive promoter from Arabidopsis thaliana (L.) Heynh. have been cloned by PCR procedure. A plant expression vector with TPS under control of Prd29A has been constructed and used for the genetic transformation of tobacco. The transgenic tobacco with Prd29A/TSP demonstrated TPS expression with increased drought tolerance under drought stress. Some obvious morphological changes including dwarf and fine shoot, lancet-shaped leaves and vigorous auxiliary buds have been observed in a few transformed plants.
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收藏
页码：616 / 619
页数：4
相关论文
共 9 条
  • [1] AUSUBEL F, 1998, SHORT PROTOCOLS MOL, P35
  • [2] CLARK MS, 1998, PLANT MOL BIOL LAB M, P333
  • [3] Inhibition of trehalase activity enhances trehalose accumulation in transgenic plants
    Goddijn, OJM
    Verwoerd, TC
    Voogd, E
    Krutwagen, PWHH
    deGraaf, PTHM
    Poels, J
    vanDun, K
    Ponstein, AS
    Damm, B
    Pen, J
    [J]. PLANT PHYSIOLOGY, 1997, 113 (01) : 181 - 190
  • [4] Drought tolerance in tobacco
    Holmstrom, KO
    Mantyla, E
    Welin, B
    Mandal, A
    Palva, ET
    [J]. NATURE, 1996, 379 (6567) : 683 - 684
  • [5] A SIMPLE AND GENERAL-METHOD FOR TRANSFERRING GENES INTO PLANTS
    HORSCH, RB
    FRY, JE
    HOFFMANN, NL
    EICHHOLTZ, D
    ROGERS, SG
    FRALEY, RT
    [J]. SCIENCE, 1985, 227 (4691) : 1229 - 1231
  • [6] IMPROVED PERFORMANCE OF TRANSGENIC FRUCTAN-ACCUMULATING TOBACCO UNDER DROUGHT STRESS
    PILONSMITS, EAH
    EBSKAMP, MJM
    PAUL, MJ
    JEUKEN, MJW
    WEISBEEK, PJ
    SMEEKENS, SCM
    [J]. PLANT PHYSIOLOGY, 1995, 107 (01) : 125 - 130
  • [7] Expression of the yeast trehalose-6-phosphate synthase gene in transgenic tobacco plants: Pleiotropic phenotypes include drought tolerance
    Romero, C
    Belles, JM
    Vaya, JL
    Serrano, R
    CulianezMacia, FA
    [J]. PLANTA, 1997, 201 (03) : 293 - 297
  • [8] CHARACTERIZATION OF THE EXPRESSION OF A DESICCATION-RESPONSIVE RD29 GENE OF ARABIDOPSIS-THALIANA AND ANALYSIS OF ITS PROMOTER IN TRANSGENIC PLANTS
    YAMAGUCHISHINOZAKI, K
    SHINOZAKI, K
    [J]. MOLECULAR & GENERAL GENETICS, 1993, 236 (2-3): : 331 - 340
  • [9] A NOVEL CIS-ACTING ELEMENT IN AN ARABIDOPSIS GENE IS INVOLVED IN RESPONSIVENESS TO DROUGHT, LOW-TEMPERATURE, OR HIGH-SALT STRESS
    YAMAGUCHISHINOZAKI, K
    SHINOZAKI, K
    [J]. PLANT CELL, 1994, 6 (02) : 251 - 264
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