Gancochlearols E - I, meroterpenoids from Ganoderma cochlear against COX-2 and triple negative breast cancer cells and the absolute configuration assignment of ganomycin K

被引：13

作者：

Li, Yan-Peng ^{[1
]}

Jiang, Xiao-Ting ^{[1
]}

Qin, Fu-Ying ^{[1
]}

Zhang, Hao-Xing ^{[1
]}

Cheng, Yong-Xian ^{[1
,2
]}

机构：

[1] Shenzhen Univ, Coll Life Sci & Oceanog, Sch Pharmaceut Sci, Shenzhen 518060, Peoples R China

[2] Shenzhen Univ, Marshall Lab Biomed Engn, Shenzhen 518060, Peoples R China

来源：

BIOORGANIC CHEMISTRY | 2021年 / 109卷

基金：

中国国家自然科学基金;

关键词：

Ganoderma cochlear; Meroterpenoids; COX-2; TNBC; EMT; TRITERPENOIDS;

D O I：

10.1016/j.bioorg.2021.104706

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Five new meroterpenoids, gancochlearols E - I (1, 3-6), and one compound ganomycin K (2) were isolated from the fruiting bodies of G. cochlear. Their structures were assigned by 1D and 2D NMR, MS, and CD analysis. Rh-2(OCOCF3)(4)-induced ECD method was used to clarify the absolute configuration of secondary alcohol in 1 and 2. Biochemical evaluation showed that all the isolates significantly inhibit COX-2 enzyme in vitro with the IC50 values range from 1.03 mu M to 2.71 mu M. Further cellular assay revealed that (+)-3 and (-)-6 could suppress metastatic phenotype of triple-negative breast cancer (TNBC) cells via impeding the epithelial-mesenchymal transition (EMT).

引用

页数：7

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