Acetic acid improves the sensitivity of theophylline analysis by gas chromatography-mass spectrometry

被引：35

作者：

Saka, Kanju ^{[1
]}

Uemura, Koichi ^{[1
]}

Shintani-Ishida, Kaori ^{[1
]}

Yoshida, Ken-ichi ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Med, Dept Forens Med, Bunkyo Ku, Tokyo 1130033, Japan

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2007年 / 846卷 / 1-2期

关键词：

theophylline; acetic acid; gas chromatography-mass spectrometry;

D O I：

10.1016/j.jchromb.2006.09.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In the analysis of theophylline by gas chromatography-mass spectrometry (GC-MS), we found that the addition of acetic acid to the solvent (ethyl acetate) decreased the adsorption of theophylline to the glass wool packed into the inlet liner. The addition of acetic acid to ethyl acetate improved the sensitivity for theophylline (optimum concentration of 3%). This simple and sensitive method without derivatization can be applied to the quantification of theophylline in serum samples in clinical and toxicological practice. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：240 / 244

页数：5

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