A first-generation EST RH comparative map of the porcine and human genome

被引：77

作者：

Rink, A ^{[1
]}

Santschi, EM

Eyer, KM

Roelofs, B

Hess, M

Godfrey, M

Karajusuf, EK

Yerle, M

Milan, D

Beattie, CW

机构：

[1] Univ Nevada, Coll Agr Biotechnol & Nat Resources, Dept Anim Biotechnol, Reno, NV 89557 USA

[2] Univ Wisconsin, Sch Vet Med, Dept Surg Sci, Madison, WI 53706 USA

[3] INRA, Lab Genet Cellulaire, F-31326 Castanet Tolosan, France

来源：

MAMMALIAN GENOME | 2002年 / 13卷 / 10期

关键词：

D O I：

10.1007/s00335-002-2192-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have constructed a first-generation EST radiation hybrid comparative map of the porcine genome by assigning 1058 markers to the IMpRH(7000) panel. Chromosomal localization was determined with a 2pt LOD of 4.8 for 984 markers, using the IMpRH mapping tool. Annotated ESTs represent 46.2% or 489 of the markers. Marker distribution was not stochastic and ranged from 0.41 for SSC8 to 1.77 for SSC12, respectively. Two hundred fifty-one markers assigned to the physical map of the pig did not find a homologous sequence in V22 of the human genome assembly, indicative of gaps in the assembled human genome sequence. The comparative porcine/human map covers 3290 MB, or 98.3% of the presumed size of the human genome. However, 60 breakpoints were identified between chromosomes, as well as 90 micro-rearrangements within synteny groups. Six porcine chromosomes-SSC2, 5, 6, 7, 12, and 14-correspond to the three gene-richest human chromosomes, HSA17, 19, and 22, and show above average marker density. Porcine Chrs 1, 87 11. and X display a low DNA/marker ratio and correspond to the 'genome deserts' on HSA 18, 4, 13, and X.

引用

页码：578 / 587

页数：10

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