Generation of Induced Pluripotent Stem Cells from Human Cord Blood

被引：375

作者：

Haase, Alexandra ^{[1
]}

Olmer, Ruth ^{[1
]}

Schwanke, Kristin ^{[1
]}

Wunderlich, Stephanie ^{[1
]}

Merkert, Sylvia ^{[1
]}

Hess, Christian ^{[1
]}

Zweigerdt, Robert ^{[1
]}

Gruh, Ina ^{[1
]}

Meyer, Johann ^{[2
]}

Wagner, Stefan ^{[7
]}

Maier, Lars S. ^{[7
]}

Han, Dong Wook ^{[6
]}

Glage, Silke ^{[3
]}

Miller, Konstantin ^{[4
]}

Fischer, Philipp ^{[5
]}

Schoeler, Hans R. ^{[6
]}

Martin, Ulrich ^{[1
]}

机构：

[1] Hannover Med Sch, Dept Cardiac Thorac Transplantat & Vasc Surg, Leibniz Res Labs Biotechnol & Artificial Organs, D-30625 Hannover, Germany

[2] Hannover Med Sch, Dept Expt Hematol, D-30625 Hannover, Germany

[3] Hannover Med Sch, Inst Lab Anim Sci, D-30625 Hannover, Germany

[4] Hannover Med Sch, Dept Human Genet, D-30625 Hannover, Germany

[5] Hannover Med Sch, Dept Cardiol & Angiol, D-30625 Hannover, Germany

[6] Max Planck Inst Mol Biomed, Dept Cell & Dev Biol, D-48149 Munster, Germany

[7] Univ Gottingen, Dept Cardiol & Pneumol, D-37075 Gottingen, Germany

来源：

CELL STEM CELL | 2009年 / 5卷 / 04期

关键词：

STEMCELL;

D O I：

10.1016/j.stem.2009.08.021

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Induced pluripotent stem cells (iPSCs) may represent an ideal cell source for future regenerative therapies. A critical issue concerning the clinical use of patient-specific iPSCs is the accumulation of mutations in somatic (stem) cells over an organism's lifetime. Acquired somatic mutations are passed onto iPSCs during reprogramming and may be associated with loss of cellular functions and cancer formation. Here we report the generation of human iPSCs from cord blood (CB) as a juvenescent cell source. CBiPSCs show characteristics typical of embryonic stem cells and can be differentiated into derivatives of all three germ layers, including functional cardiomyocytes. For future therapeutic production of autologous and allogeneic iPSC derivatives, CB could be routinely harvested for public and commercial CB banks without any donor risk. CB could readily become available for pediatric patients and, in particular, for newborns with genetic diseases or congenital malformations.

引用

页码：434 / 441

页数：8

共 14 条

[1] Mobilizing stem cells from normal donors: is it possible to improve upon G-CSF? [J].