Detection of human papillomavirus in oropharyngeal squamous cell carcinoma

被引:15
|
作者
Fauzi, Fatin Hazwani [1 ]
Hamzan, Nurul Izzati [1 ]
Rahman, Nurhayu Ab [1 ]
Suraiya, Siti [2 ]
Mohamad, Suharni [1 ]
机构
[1] Univ Sains Malaysia, Sch Dent Sci, Kubang Kerian 16150, Kelantan, Malaysia
[2] Univ Sains Malaysia, Sch Med Sci, Dept Med Microbiol & Parasitol, Kubang Kerian 16150, Kelantan, Malaysia
来源
关键词
Human papillomavirus (HPV); Molecular detection; Oropharyngeal squamous cell carcinoma (OPSCC); R739; 63; MEDIATED ISOTHERMAL AMPLIFICATION; IN-SITU HYBRIDIZATION; E6/E7; MESSENGER-RNA; HIGH VIRAL LOAD; RAPID DETECTION; P16; IMMUNOHISTOCHEMISTRY; NECK-CANCER; NESTED-PCR; VIRUS HPV; HEAD;
D O I
10.1631/jzus.B2000161
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Worldwide there has been a significant increase in the incidence of oropharyngeal squamous cell carcinoma (OPSCC) etiologically attributed to oncogenic human papillomavirus (HPV). Reliable and accurate identification and detection tools are important as the incidence of HPV-related cancer is on the rise. Several HPV detection methods for OPSCC have been developed and each has its own advantages and disadvantages in regard to sensitivity, specificity, and technical difficulty. This review summarizes our current knowledge of molecular methods for detecting HPV in OPSCC, including HPV DNA/RNA polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), p16 immunohistochemistry (IHC), and DNA/RNA in situ hybridization (ISH) assays. This summary may facilitate the selection of a suitable method for detecting HPV infection, and therefore may help in the early diagnosis of HPV-related carcinoma to reduce its mortality, incidence, and morbidity.
引用
收藏
页码:961 / 976
页数:16
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