Assembling Human Platelet Lysate into Multiscale 3D Scaffolds for Bone Tissue Engineering

被引：21
作者：
Oliveira, Sara M. [1 ,2 ]
Reis, Rui L. [1 ,2 ]
Mano, Joao F. [1 ,2 ]
机构：
[1] Univ Minho, Dept Polymer Engn, Res Grp Biomat Biodegradables & Biomimet 3Bs, AvePk Taipas, P-4806909 Guimaraes, Portugal
[2] ICVS 3Bs PT Govt Associate Lab, P-4806909 Braga, Portugal
来源：
ACS BIOMATERIALS SCIENCE & ENGINEERING | 2015年 / 1卷 / 01期
基金：
欧洲研究理事会;
关键词：
platelet lysate; layer-by-layer assembling; osteogenesis; multiscale scaffold; instructive; FIBROBLAST-GROWTH-FACTOR; RICH PLASMA; OSTEOGENIC DIFFERENTIATION; HEPARAN-SULFATE; STEM-CELLS; DEXAMETHASONE; TRANSCRIPTION; ENHANCEMENT; OSTEOBLASTS; DELIVERY;
D O I：
10.1021/ab500006x
中图分类号：
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号：
0805 ; 080501 ; 080502 ;
摘要：
Scaffolds for bone tissue engineering lack often control of cellular instructions. We propose a triple sequential approach for customizing scaffold features from the macro to the nanoscale. The nano/mesoscale is composed by human platelet lysate and marine-origin polysaccharides assembled by layer-by-layer and shaped into fibrils by freeze-drying. We show that osteogenic induction of stem cells is tunable within a low range of layers. This approach has the potential to develop new scaffolds with enhanced cell-instructive capabilities using affordable autologous sources of bioactive molecules.
引用
收藏
页码：2 / 6
页数：5
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