Production of transgenic plants via Agrobacterium rhizogenes-mediated transformation of Panax ginseng

被引:45
作者
Yang, DC [1 ]
Choi, YE [1 ]
机构
[1] Korea Ginseng & Tobacco Res Inst, Taejon 305345, South Korea
关键词
Panax ginseng; hairy root; plant regeneration;
D O I
10.1007/s002990050761
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Hairy roots of Panax ginseng were obtained after root disks were infected with wild-type strain Agrobacterium rhizogenes 15834. Three lines of hairy roots with different pigmentation were selected. Embryogenic callus was induced on Murashige and Skoog medium containing 1.0 mg/l 2,4-D. The frequency of embryogenic callus formation was 37.4% in a line with red pigmentation. Somatic embryo development from embryogenic callus was efficiently achieved by lowering the concentration of 2,4-D (0.5 mg/l). After the germination of somatic embryos on medium with 10 mg/l GA(3), the embryos were transferred to 1/2-MS medium without GA(3). The transformed ginseng plantlets had an actively growing root system with abundant lateral roots. The phenotypical alteration of transformed ginseng plants might be valuable character for increasing root yield.
引用
收藏
页码:491 / 496
页数:6
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