Involvement of two rice ETHYLENE INSENSITIVE3-LIKE genes in wound signaling

被引：44

作者：

Hiraga, Susumu ^{[2
]}

Sasaki, Katsutomo ^{[1
]}

Hibi, Tadaharu ^{[3
]}

Yoshida, Hitoshi ^{[3
]}

Uchida, Eiji ^{[3
]}

Kosugi, Shunichi ^{[4
]}

Kato, Takeshi ^{[5
]}

Mie, Takashi ^{[5
]}

Ito, Hiroyuki ^{[5
]}

Katou, Shinpei ^{[1
]}

Seo, Shigemi ^{[1
]}

Matsui, Hirokazu ^{[5
]}

Ohashi, Yuko ^{[1
]}

Mitsuhara, Ichiro ^{[1
]}

机构：

[1] NIAS, Tsukuba, Ibaraki 3058602, Japan

[2] Natl Agr & Food Res Org, NICS, Tsukuba, Ibaraki 3058518, Japan

[3] NARO, Natl Agr Res Ctr, Hokuriku Res Ctr, Niigata 9430193, Japan

[4] Nara Inst Sci & Technol, Grad Sch Biol Sci, Nara 6300101, Japan

[5] Hokkaido Univ, Div Appl Biosci, Grad Sch Agr, Sapporo, Hokkaido 0608589, Japan

来源：

MOLECULAR GENETICS AND GENOMICS | 2009年 / 282卷 / 05期

关键词：

DNA-binding specificity; EHTYLENE INSENSITIVE 3 (EIN3); Tobacco EIN3 like (TEIL); TEIL binding site (tebs); Microarray analysis; Gene expression; Rice (Oryza sativa L.); Transcriptional activation; Wounding; TOBACCO PEROXIDASE GENE; DNA-BINDING DOMAIN; ARABIDOPSIS-THALIANA; POSITIVE COMPONENT; FLOWER DEVELOPMENT; PLANT DEVELOPMENT; VASCULAR SYSTEM; JASMONIC ACID; BLAST FUNGUS; CIS-ELEMENT;

D O I：

10.1007/s00438-009-0483-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ethylene and jasmonic acid (JA) have been proposed as key compounds for wound signaling in plants. In Arabidopsis, ETHYLENE INSENSITIVE3 (EIN3), which is an essential transcription factor for ethylene signaling, is regulated at the post-transcriptional level, while transcriptional regulation of EIN3 or EIN3-LIKE (EIL) genes has not been well documented. The expression of 6 rice EIL genes (OsEIL1-6) was analyzed and only OsEIL1 and 2 were found to be wound-inducible EIL. OsEIL2 was also induced by JA. Electrophoretic mobility shift assays showed that recombinant OsEIL1 and 2 proteins bound to specific DNA sequences that are recognized by a wound-inducible tobacco EIL. Accumulation of OsEIL1 and 2 transcripts reached a maximum at 1 and 0.5 h after wounding, respectively, and the corresponding DNA-binding activity in nuclear extracts of rice leaves was increased at 1 h after wounding. Candidates for OsEIL-target genes were selected by microarray analysis of wounded rice and by promoter sequence analyses of wound-inducible genes identified by microarray analysis. In OsEIL1- and/or 2-suppressed rice plants, the expression of at least four of 18 candidate genes analyzed was down-regulated. These results indicate the importance of inducible OsEILs in wound signaling in rice.

引用

页码：517 / 529

页数：13

共 49 条

[1] RICE ASPARTIC PROTEINASE, ORYZASIN, EXPRESSED DURING SEED RIPENING AND GERMINATION, HAS A GENE ORGANIZATION DISTINCT FROM THOSE OF ANIMAL AND MICROBIAL ASPARTIC PROTEINASES [J].