Development of protoplasts of Ulva fasciata (Chlorophyta) for algal seed stock

The aim of this study was to isolate and cultivate protoplasts of the green alga Ulva fasciata Delile and subsequently induce them to form a microthallus suspension for algal seed stock. The protoplasts were covered with secreted mucilage following 6 h of culture when viewed with SEM, The mucilage fused to form thick layers during day 1 of culture. Microfibrillar cell walls were deposited into the thick layers of mucilage on the 5th day of culture. An average of about 10% of the freshly belated protoplasts began to divide at 6-14 days. These protoplasts subsequently developed varied morphologies, depending on the time of collection during the year. Protoplasts isolated from U, fasciata collected in March to June developed frond thalli or microthalli when they were cultured in low or high densities (cells/area), respectively, The microthallus suspension was cultured for more than two years at 10-40 mu mol(.)m(-2.)s(-1). Frond thalli Formed when the suspension was cultivated at 100-160 mu mol(.)m(-2.)s(-1) Therefore, microthallus suspension can serve as a seed stock of C. fasciata.