A highly sensitive, non-radioactive assay for T cell activation in cattle:: applications in screening for antigens recognised by CD4+ and CD8+ T cells

被引：9

作者：

Ballingall, KT

Mwangi, DM

MacHugh, ND

Taracha, ELN

Totte, P

McKeever, DJ

机构：

[1] Int Livestock Res Inst, Nairobi, Kenya

[2] CIRAD, EMVT, F-97165 Pointe A Pitre, Guadeloupe, France

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2000年 / 239卷 / 1-2期

关键词：

endothelial cells; MHC; T cell activation; bovine;

D O I：

10.1016/S0022-1759(00)00187-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a highly sensitive, non-radioactive assay for T cell activation, based on the rapid induction of class II MHC expression by constitutively negative bovine endothelial cells, when cultured in the presence of supernatants derived from activated bovine T cells. We demonstrate the effectiveness of this assay in detecting rBoIFN gamma and activation of immune CD4(+) and CD8(+) T cell lines and clones in response to specific antigen and transfected COS-7 cells, respectively. We also demonstrate its utility in identifying purified pathogen fractions that activate immune CD4(-) T cell clones. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：85 / 93

页数：9

共 37 条

[1] A SIMPLE NEW METHOD FOR USING ANTIGENS SEPARATED BY POLYACRYLAMIDE-GEL ELECTROPHORESIS TO STIMULATE LYMPHOCYTES INVITRO AFTER CONVERTING BANDS CUT FROM WESTERN BLOTS INTO ANTIGEN-BEARING PARTICLES [J].