Dual symbiosis between Piriformospora indica and Azotobacter chroococcum enhances the artemisinin content in Artemisia annua L.

被引:50
作者
Arora, Monika [1 ]
Saxena, Parul [2 ]
Choudhary, Devendra Kumar [1 ]
Abdin, Malik Zainul [2 ]
Varma, Ajit [1 ]
机构
[1] Amity Inst Microbial Technol, Block E-3,4th Floor,Amity Univ Campus,Sect 125, Noida 201313, UP, India
[2] Hamdard Univ, Fac Sci, Ctr Transgen Plant Dev, New Delhi, India
关键词
Artemisia annua; Artemisinin; Artemisinin biosynthesis genes; Azotobacter chroococcum (W-5); Dual symbiosis; Piriformospora indica; ARBUSCULAR MYCORRHIZAL FUNGI; ANTIMALARIAL-DRUG; PLANT-GROWTH; ROOTS; COLONIZATION; NITROGEN; BIOSYNTHESIS; CHLOROPHYLL; PRECURSOR; BACTERIA;
D O I
10.1007/s11274-015-1972-5
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
At present, Artemisia annua L. is the major source of artemisinin production. To control the outbreaks of malaria, artemisinin combination therapies (ACTs) are recommended, and hence an ample amount of artemisinin is required for ACTs manufacture to save millions of lives. The low yield of this antimalarial drug in A. annua L. plants (0.01-1.1 %) ensues its short supply and high cost, thus making it a topic of scrutiny worldwide. In this study, the effects of root endophyte, Piriformospora indica strain DSM 11827 and nitrogen fixing bacterium, Azotobacter chroococcum strain W-5, either singly and/or in combination for artemisinin production in A. annua L. plants have been studied under poly house conditions. The plant growth was monitored by measuring parameters like height of plant, total dry weight and leaf yield with an increase of 63.51, 52.61 and 79.70 % respectively, for treatment with dual biological consortium, as compared to that of control plants. This significant improvement in biomass was associated with higher total chlorophyll content (59.29 %) and enhanced nutrition (especially nitrogen and phosphorus, 55.75 and 86.21 % respectively). The concentration of artemisinin along with expression patterns of artemisinin biosynthesis genes were appreciably higher in dual treatment, which showed positive correlation. The study suggested the potential use of the consortium P. indica strain DSM 11827 and A. chroococcum strain W-5 in A. annua L. plants for increased overall productivity and sustainable agriculture.
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页数:10
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共 53 条
  • [51] RETRACTED: A Phosphate Transporter from the Root Endophytic Fungus Piriformospora indica Plays a Role in Phosphate Transport to the Host Plant (Retracted article. See vol. 296, 2021)
    Yadav, Vikas
    Kumar, Manoj
    Deep, Deepak Kumar
    Kumar, Hemant
    Sharma, Ruby
    Tripathi, Takshashila
    Tuteja, Narendra
    Saxena, Ajay Kumar
    Johri, Atul Kumar
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285 (34) : 26532 - 26544
  • [52] Adherence to Artemisinin-Based Combination Therapy for the Treatment of Uncomplicated Malaria: A Systematic Review and Meta-Analysis
    Yakasai, Ahmad M.
    Hamza, Muhammad
    Dalhat, Mahmood M.
    Bello, Musa
    Gadanya, Muktar A.
    Yaqub, ZuwairaM.
    Ibrahim, Daiyabu A.
    Hassan-Hanga, Fatimah
    [J]. JOURNAL OF TROPICAL MEDICINE, 2015, 2015
  • [53] Zhao SS, 1986, J PHARM ANAL, V6, P3