Cobalt(II)-Responsive DNA Binding of a GCN4-bZIP Protein Containing Cysteine Residues Functionalized with Iminodiacetic Acid

被引：18

作者：

Azuma, Yusuke ^{[1
]}

Imanishi, Miki ^{[1
]}

Yoshimura, Tomoyuki ^{[1
]}

Kawabata, Takeo ^{[1
]}

Futaki, Shiroh ^{[1
]}

机构：

[1] Kyoto Univ, Inst Chem Res, Kyoto 6110011, Japan

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 37期

关键词：

biosensors; DNA binding; helix destabilization; metal switches; proteins; COILED-COIL; PEPTIDE; PHOTOCONTROL; TRANSITION; HELICES; DOMAIN; DIMER; GCN4;

D O I：

10.1002/anie.200902888

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Two pairs to win: Two pairs of iminodiacetic acid (Ida) moieties were incorporated in the leucine zipper segment of the GCN4-bZIP protein in such a way that the Ida moieties of each pair were in i and i+2 positions. Complex formation of the Ida groups with CoII led to destabilization of the helical structure and thus enabled reversible switching of the binding of the protein to the target AP-1 site (see picture). © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：6853 / 6856

页数：4

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