How xenopus laevis replicates DNA reliably even though its origins of replication are located and initiated stochastically

被引：27

作者：

Bechhoefer, John ^{[1
]}

Marshall, Brandon ^{[1
]}

机构：

[1] Simon Fraser Univ, Dept Phys, Burnaby, BC V5A 1S6, Canada

来源：

PHYSICAL REVIEW LETTERS | 2007年 / 98卷 / 09期

关键词：

D O I：

10.1103/PhysRevLett.98.098105

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

DNA replication in Xenopus laevis is extremely reliable, failing to complete before cell division no more than once in 10 000 times; yet replication origin sites are located and initiated stochastically. Using a model based on 1D theories of nucleation and growth and using concepts from extreme-value statistics, we derive the distribution of replication times given a particular initiation function. We show that the experimentally observed initiation strategy for Xenopus laevis meets the reliability constraint and is close to the one that requires the fewest resources of a cell.

引用

页数：4

共 24 条

[1] Nucleation and growth in one dimension [J].

BenNaim, E ;

Krapivsky, PL .

PHYSICAL REVIEW E, 1996, 54 (04) :3562-3568

[2] Replication origins in Xenopus egg extract are 5-15 kilobases apart and are activated in clusters that fire at different times [J].

Blow, JJ ;

Gillespie, PJ ;

Francis, D ;

Jackson, DA .

JOURNAL OF CELL BIOLOGY, 2001, 152 (01) :15-25

[3] Control of chromosomal DNA replication in the early Xenopus embryo [J].

Blow, JJ .

EMBO JOURNAL, 2001, 20 (13) :3293-3297

[4]

Christian J., 2002, THEORY PHASE TRANS 1

[5]

Gumbel E. J., 1958, Statistics of Extremes

[6] Programmed cell death during Xenopus development:: A spatio-temporal analysis [J].

Hensey, C ;

Gautier, J .

DEVELOPMENTAL BIOLOGY, 1998, 203 (01) :36-48

[7] Replication fork density increases during DNA synthesis in X-laevis egg extracts [J].

Herrick, J ;

Stanislawski, P ;

Hyrien, O ;

Bensimon, A .

JOURNAL OF MOLECULAR BIOLOGY, 2000, 300 (05) :1133-1142

[8] Kinetic model of DNA replication in eukaryotic organisms [J].

Herrick, J ;

Jun, S ;

Bechhoefer, J ;

Bensimon, A .

JOURNAL OF MOLECULAR BIOLOGY, 2002, 320 (04) :741-750

[9] Paradoxes of eukaryotic DNA replication: MCM proteins and the random completion problem [J].

Hyrien, O ;

Marheineke, K ;

Goldar, A .

BIOESSAYS, 2003, 25 (02) :116-125

[10] CHROMOSOMAL REPLICATION INITIATES AND TERMINATES AT RANDOM SEQUENCES BUT AT REGULAR INTERVALS IN THE RIBOSOMAL DNA OF XENOPUS EARLY EMBRYOS [J].

HYRIEN, O ;

MECHALI, M .

EMBO JOURNAL, 1993, 12 (12) :4511-4520

← 1 2 3 →