Involvement of orexin-A on micturition reflex in normal and cyclophosphamide-induced cystitis bladder in rat

被引：10
作者：
Kobayashi, Mizuki [1 ,2 ]
Nomura, Masayoshi [2 ]
Fujihara, Hiroaki
Suzuki, Hitoshi
Otsubo, Hiroki [2 ]
Nishii, Hisae [2 ]
Fujimoto, Naohiro [2 ]
Matsumoto, Tetsuro [2 ]
Ueta, Yoichi
机构：
[1] Univ Occupat & Environm Hlth, Sch Med, Dept Physiol, Yahatanishi Ku, Kitakyushu, Fukuoka 8078555, Japan
[2] Univ Occupat & Environm Hlth, Sch Med, Dept Urol, Kitakyushu, Fukuoka 8078555, Japan
来源：
PEPTIDES | 2009年 / 30卷 / 12期
关键词：
Bladder; Micturition reflex; Orexin-A; Orexin-1; receptor; Spinal cord; SPINAL-CORD; HYPOCRETIN OREXIN; HYPOTHALAMIC AREA; GENE-EXPRESSION; NITRIC-OXIDE; RECEPTORS; PEPTIDES; PAIN; BRAIN; MODEL;
D O I：
10.1016/j.peptides.2009.07.025
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The purpose of the present study was to investigate the effect of orexin-A in the spinal cord on bladder function in normal rats and cyclophosphamide (CYP)-induced cystitis rat models. The effects of intrathecal (i.t.) injection of orexin-A (0.01, 0.1 and 1.0 nmol) on bladder function were examined during continuous infusion cystometrogram (CMG) in urethane anesthetized normal and CYP-induced cystitis rats. The effects of i.t. injection of selective orexin-1 receptor (OXR1) antagonist SB334867 (10 nmol) on orexin-A-induced bladder overactivity in normal rats and SB334867 (10 and 30 nmol) on changes in bladder function in normal and CYP-induced cystitis rats were investigated. The effects of intravenous (i.v.) injection of orexin-A (0.3 and 1.0 nmol) on micturition reflex were also investigated in normal rats. I.t. injection of orexin-A (0.1 and 1.0 nmol) significantly decreased the intercontraction intervals (ICI) in normal and CYP-induced cystitis rats. I.t. injection of SB334867 (10 nmol) significantly increased the ICI of orexin-A induced overactive bladder in normal rats and I.t. injection of SB334867 (30 nmol) also increased the ICI in normal rat bladder. However, in CYP-injected cystitis rat models, i.t. injection of SB334867 did not change the bladder function. I.v. injection of orexin-A failed to affect the bladder function in normal rats. Orexin mRNA levels in the lateral hypothalamus were significantly decreased in CYP-induced cystitis rats. These results indicate that orexin-A in the spinal cord activates micturition reflex via OXR1 in normal rats. In addition, OXR1 antagonist did not have any effect on micturition reflex in CYP-induced cystitis rats. (C) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码：2348 / 2356
页数：9
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