Stereochemical features of C-methylations on the path to Delta 24(28)-methylene and Delta(24(28))-ethylidene sterols: Studies on the recombinant phytosterol methyl transferase from Arabidopsis thaliana

被引：0

作者：

Tong, Y ^{[1
]}

McCourt, BS ^{[1
]}

Guo, DA ^{[1
]}

Mangla, AT ^{[1
]}

Zhou, WX ^{[1
]}

Jenkins, MD ^{[1
]}

Zhou, W ^{[1
]}

Lopez, M ^{[1
]}

Nes, WD ^{[1
]}

机构：

[1] TEXAS TECH UNIV,DEPT CHEM & BIOCHEM,LUBBOCK,TX 79409

来源：

TETRAHEDRON LETTERS | 1997年 / 38卷 / 35期

关键词：

D O I：

暂无

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Using a homogenate prepared from Escherichia coli cells that express the sterol methyl transferase (Sh IT) gene of Arabidopsis thaliana, migration of the hydrogen atom at C-24 to C-25 from the Re-face of the double bond was demonstrated in the biosynthesis of [27-C-13] 24(28)-methylenezymosterol (fecosterol) from [27-C-13]zymosterol and the chirality of the C-25 stereocenter (25R) was found to be retained after the stereospecific conversion of [27-C-13]24(28)-methylenezymosterol to [27-C-13](24(28)Z) -ethylidenecholest-8-en-3 beta-ol. (C) 1997 Elsevier Science Ltd.

引用

页码：6115 / 6118

页数：4

共 5 条

[1] Stereochemical Features of C-methylations on the Path to cap delta24(28)-Methylene and cap delta24(28)-Ethylidene Sterols: Studies on the Recombinant Phytosterol Methyl Transferase from Arabidopsis thaliana
Tong, Y.
McCourt, B. S.
Guo, D.-A.
Mangla, A. T.
Tetrahedron Letters, 38 (35):
[2] Substrate-based inhibitors of the (S)-adenosyl-L-methionine:Delta(24(25))- to Delta(24(28))-sterol methyl transferase from Saccharomyces cerevisiae
Nes, WD
Guo, DA
Zhou, W
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1997, 342 (01) : 68 - 81
[3] Overexpression, purification, and stereochemical studies of the recombinant (S)-adenosyl-L-methionine:: Δ24(25)- to Δ24(28)-sterol methyl transferase enzyme from Saccharomyces cerevisiae
Nes, WD
McCourt, BS
Zhou, WX
Ma, JZ
Marshall, JA
Peek, LA
Brennan, M
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1998, 353 (02) : 297 - 311
[4] Substrate-Based Inhibitors of the (S)-Adenosyl-L-Methionine:cap delta24(25)- to cap delta 24(28)-Sterol Methyl Transferase from Saccharomyces cerevisiae
Nes, W. D.
Guo, D.-A.
Zhou, W.
Archives of Biochemistry and Biophysics, 342 (01):
[5] A NEW C-30 STEROL, (24Z)-24-PROPYLCHOLESTA-5,7,24(28)-TRIEN-3-BETA-OL, AND OTHER DELTA-5,7 STEROLS FROM THE SPONGE SPONGIONELLA-GRACILIS
SICA, D
PICCIALLI, V
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1985, 81 (01): : 115 - 118

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