DNA Damage/Repair and Polymorphism of the hOGG1 Gene in Lymphocytes of AMD Patients

被引：20

作者：

Wozniak, Katarzyna ^{[1
]}

Szaflik, Jacek P. ^{[2
]}

Zaras, Malgorzata ^{[2
]}

Sklodowska, Anna ^{[2
]}

Janik-Papis, Katarzyna ^{[1
]}

Poplawski, Tomasz R. ^{[1
]}

Blasiak, Janusz ^{[1
]}

Szaflik, Jerzy ^{[2
]}

机构：

[1] Univ Lodz, Dept Mol Genet, PL-90237 Lodz, Poland

[2] Med Univ Warsaw, Dept Ophthalmol, PL-03709 Warsaw, Poland

来源：

JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY | 2009年

关键词：

RETINAL-PIGMENT EPITHELIUM; COMPLEMENT FACTOR-H; AGE-RELATED MACULOPATHY; BASE EXCISION-REPAIR; MACULAR DEGENERATION; OXIDATIVE-STRESS; CANCER RISK; SER326CYS POLYMORPHISM; HUMAN-CELLS; ASSOCIATION;

D O I：

10.1155/2009/827562

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Oxidative stress is thought to play a role in the pathogenesis of age-related macular degeneration (AMD). We determined the extent of oxidative DNA damage and the kinetics of its removal as well as the genotypes of the Ser326Cys polymorphism of the hOGG1 gene in lymphocytes of 30 wet AMD patients and 30 controls. Oxidative DNA damage induced by hydrogen peroxide and its repair were evaluated by the comet assay and DNA repair enzymes. We observed a higher extent of endogenous oxidative DNA damage and a lower efficacy of its repair in AMD patients as compared with the controls. We did not find any correlation between the extent of DNA damage and efficacy of DNA repair with genotypes of the Ser326Cys polymorphism. The results obtained suggest that oxidative DNA damage and inefficient DNA repair can be associated with AMD and the variability of the hOOG1 gene may not contribute to this association. Copyright (C) 2009 Katarzyna Wozniak et al.

引用

页数：9

共 46 条

[1] Prevalence of age-related maculopathy in older Europeans - The European Eye Study (EUREYE) [J].