Evaluation of a new real-time PCR assay for the direct detection of diarrheagenic Escherichia coli in stool specimens

被引：13
作者：
Eigner, U. [1 ]
Hiergeist, A. [2 ]
Veldenzer, A. [1 ]
Rohlfs, M. [1 ]
Schwarz, R. [1 ]
Holfelder, M. [1 ]
机构：
[1] Lab Limbach Heidelberg, Heidelberg, Germany
[2] Univ Hosp Regensburg, Inst Clin Microbiol & Hyg, Regensburg, Germany
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2017年 / 88卷 / 01期
关键词：
Diarrheagenic E. coli; RIDA (R) GENE (RG) real-time multiplex PCR assays; Shiga toxin-producing E. coli (STEC); Enteropathogenic E. coil (EPEC); Enteroaggregative E. coli (EAEC); Enterotoxinogenic E. coli (ETEC); Enteroinvasive E. coli (EIEC); MULTIPLEX PCR; INFECTION; PATHOGENS; SAMPLES; PANEL;
D O I：
10.1016/j.diagmicrobio.2017.01.016
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Diarrheagenic E. coli (DEC) are one of the most common causes for diarrhea worldwide, especially in children. We evaluated the rapid RIDA (R) GENE (RG) real-time multiplex PCR assays (R-Biopharm, Darmstadt, Germany) for the detection of the most important diarrheagenic E. coli. Three hundred fifteen liquid or non-formed stool specimens were examined. The results of the RG multiplex assays were compared to specific PCR methods. The sensitivity and specificity of the RG PCRs were as follows, 100%/100% for the detection of EHEC, 963% and 99% for EPEC, 100% and 100% for the detection of EAEC, ETEC and EIEC, respectively. Overall, the RG real-time PCR system for the detection of DEC tested in this study provided reliable and rapid results and shows the ability as a useful addendum for the detection of diarrheagenic E. coli in the medical laboratory. (C) 2017 Elsevier Inc. All rights reserved.
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收藏
页码：12 / 16
页数：5
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