Production, purification and characterization of pectinase from a Bacillus sp DT7

被引：112

作者：

Kashyap, DR ^{[1
]}

Chandra, S ^{[1
]}

Kaul, A ^{[1
]}

Tewari, R ^{[1
]}

机构：

[1] Panjab Univ, Dept Microbiol, Chandigarh 160014, India

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2000年 / 16卷 / 03期

关键词：

Bacillus; pectinase; production; purification;

D O I：

10.1023/A:1008902107929

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A soil isolate, Bacillus sp. DT7 has been found to produce significant amounts of an extracellular pectinase subsequently characterized as pectin lyase (EC 4.2.2.10). By optimizing growth conditions, Bacillus sp. DT7 produced higher amount of pectin lyase (53 units/ml) than that has been reported in the literature. Using gel filtration and ion exchange chromatography, this enzyme was purified and found to have a molecular mass of 106 kDa. The purified enzyme exhibited maximal activity at a temperature of 60 degrees C and pH 8.0. The presence of 100 mM concentrations of CaCl2 and mercaptoethanol significantly enhanced pectinase activity of the purified enzyme. This pectinase has tremendous applications in textile industry, plant tissue maceration and fruit juice wastewater treatments.

引用

页码：277 / 282

页数：6

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