A new type of biocompatible fluorescent probe AFN for fixed and live cell imaging of intracellular lipid droplets

被引：54

作者：

Sharma, Ashutosh ^{[1
]}

Umar, Shahida ^{[1
]}

Kar, Parmita ^{[2
]}

Singh, Kavita ^{[3
]}

Sachdev, Monika ^{[2
]}

Goel, Atul ^{[1
,4
]}

机构：

[1] Cent Drug Res Inst, CSIR, Med & Proc Chem Div, Fluorescent Lab, Lucknow 226031, Uttar Pradesh, India

[2] Cent Drug Res Inst, CSIR, Div Endocrinol, Lucknow 226031, Uttar Pradesh, India

[3] Cent Drug Res Inst, CSIR, Electron Microscopy Unit, Lucknow 226031, Uttar Pradesh, India

[4] Acad Sci & Innovat Res, New Delhi 110001, India

来源：

ANALYST | 2016年 / 141卷 / 01期

关键词：

INTRAMOLECULAR CHARGE-TRANSFER; OPTICAL-PROPERTIES; DERIVATIVES; METABOLISM; METALATION; MECHANISM; BLUE; FLUORENONES; EMISSION; MOLECULE;

D O I：

10.1039/c5an01623a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We discovered a new class of nontoxic, highly fluorogenic and biocompatible probe AFN for selective staining of intracellular Lipid Droplets (LDs) in both fixed and live human cervical cancer cells (HeLa) and 3T3-L1 pre-adipocytes without any background artifacts. The salient features of the probe are its visible excitation maximum, aqueous compatibility, selectivity and remarkable stability (for more than a week) in live cells, even better than commercially available Nile red.

引用

页码：137 / 143

页数：7

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