High-throughput protein digestion by trypsin-immobilized monolithic silica with pipette-tip formula

被引：50
作者：
Ota, Shigenori
Miyazaki, Shota
Matsuoka, Hideo
Morisato, Kei
Shintani, Yukihiro
Nakanishi, Kazuki
机构：
[1] GL Sci Inc, Iruma, Saitama 3580032, Japan
[2] Kyoto Monotech Co, Nishikyo Ku, Kyoto 6158035, Japan
[3] Kyoto Univ, Grad Sch Sci, Dept Chem, Sakyo Ku, Kyoto 6068502, Japan
来源：
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2007年 / 70卷 / 01期
关键词：
trypsin-immobilized monolithic silica; digestion tool with pipette-tip shape; high-throughput digestion; tryptic mapping; proteins;
D O I：
10.1016/j.jbbm.2006.10.005
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Based on the monolithic silica gel materials with hierarchical pore structure and on the SPE devices (MonoTip (R)) developed thereof, a trypsin-immobilized monolithic silica in a pipette tip (MonoTip (R) Trypsin) suitable for digesting proteins has been newly developed. The surface of monolithic silica fixed into the tip was chemically modified with trypsin via an aminopropyl group. Trypsin-immobilized monolith successfully performed a rapid digestion of reduced and alkylated proteins with only a few times pipetting operation for the pre-treatment procedure of chromatographic analysis. The novel solid-phase digestion tool using monolithic silica allows a high-throughput trypsin proteolysis of bio-substances in proteomics. (c) 2006 Elsevier B.V. All rights reserved.
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收藏
页码：57 / 62
页数：6
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