Bio-orthogonal Immobilization of Fibroblast Growth Factor 2 for Spatial Controlled Cell Proliferation

被引：29

作者：

Luehmann, Tessa ^{[1
]}

Jones, Gabriel ^{[1
]}

Gutmann, Marcus ^{[1
]}

Rybak, Jens-Christoph ^{[1
]}

Nickel, Joachim ^{[2
,3
]}

Rubini, Marina ^{[4
]}

Meinel, Lorenz ^{[1
]}

机构：

[1] Univ Wurzburg, Inst Pharm & Food Chem, D-97074 Wurzburg, Germany

[2] Univ Wurzburg, Chair Tissue Engn & Regenerat Med, D-97070 Wurzburg, Germany

[3] Branch Fraunhofer Inst Interfacial Engn & Biotech, Translat Ctr Regenerat Therapies Oncol & Musculos, Wurzburg, Germany

[4] Univ Konstanz, Inst Organ Chem, Constance, Germany

来源：

ACS BIOMATERIALS SCIENCE & ENGINEERING | 2015年 / 1卷 / 09期

关键词：

fibroblast growth factor 2 (FGF-2); bio-orthogonal immobilization; genetic codon expansion; decoration; proliferation; FACTOR DELIVERY; TISSUE-REPAIR; PROTEINS; MATRICES; RECEPTOR;

D O I：

10.1021/acsbiomaterials.5b00236

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

Presentation of therapeutic proteins on material surfaces is challenged by random immobilization chemistries through lysine or cysteine residues, typically leading to heterogeneous product outcome. Pharmaceutical quality standards warrant a controlled process ideally through site specific conjugation. Therefore, we deployed genetic codon expansion to engineer a propargyl-L-lysine (Plk)-modified FGF-2 analogue, enabling site-specific copper(I)-catalyzed azide alkyne cycloaddition (CuAAC). Site-specific decoration of Plk-FGF-2 to particles sparked cell proliferation of human osteosarcoma cells in a spatially controlled manner around the decorated carrier, rendering this approach instrumental for the future design of quality-improved bioinstructive scaffold outcome.

引用

页码：740 / 746

页数：7

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