Evaluation of the sensitivities of DNA extraction and PCR methods for detection of Giardia duodenalis in stool specimens

被引：40

作者：

Nantavisai, Kwannan

Mungthin, Mathirut

Tan-ariya, Peerapan

Rangsin, Ram

Naaglor, Tawee

Leelayoova, Saovanee

机构：

[1] Phramongkutklao Coll Med, Dept Parasitol, Bangkok 10400, Thailand

[2] Mahidol Univ, Fac Sci, Dept Microbiol, Bangkok 10400, Thailand

[3] Phramongkutklao Coll Med, Dept Military & Commun Med, Bangkok 10400, Thailand

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2007年 / 45卷 / 02期

关键词：

D O I：

10.1128/JCM.01823-06

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Sensitivities of DNA extraction methods and PCR methods for Giardia duodenalis were evaluated. A combination of the most sensitive methods, i.e., FTA filter paper and a PCR protocol using RH11/RH4 and GiarF/GiarR primers, showed no significant differences compared to immunofluorescence assay in terms of their sensitivities and specificities.

引用

页码：581 / 583

页数：3

共 13 条

[11] Triosephosphate isomerase gene characterization and potential zoonotic transmission of Giardia duodenalis [J].