Reduction of 4-nitrophenol catalyzed by nitroreductase

被引：16

作者：

Chen, Jie

Dai, Rong Ji ^{[1
]}

Tong, Bin

Xiao, Sheng Yuan

Meng, Wei Wei

机构：

[1] Beijing Inst Technol, Sch Life Sci & Technol, Beijing 100081, Peoples R China

[2] Beijing Inst Technol, Sch Mat Sci & Engn, Beijing 100081, Peoples R China

[3] Beijing Univ Chinese Med, Dongfang Coll, Langfang 065000, Peoples R China

来源：

CHINESE CHEMICAL LETTERS | 2007年 / 18卷 / 01期

关键词：

nitroreductase; 4-nitrophenol; biotransformation; reduction;

D O I：

10.1016/j.cclet.2006.11.009

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The reduction of 4-nitrophenol catalyzed by nitroreductase in the presence of NADH was investigated in this paper. 4-Aminophenol and 4-hydroxylamino-phenol were found in the reductive products. The relationship between reaction time and the reductive ratio were studied. The similar reducing ratios of 4-nitrophenol were obtained under aerobic and anaerobic conditions. The results indicated that an oxygen-insensitive reaction was proceeded in the reduction of 4-nitrophenol and nitroreductase was an oxygen-insensitive enzyme. The reductive products of 4-nitrophenol were determined by HPLC and MS. (c) 2006 Rong Ji Dai. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.

引用

页码：10 / 12

页数：3

共 4 条

[1] Nitroreductase catalyzed biotransformation of CL-20 [J].