Platform for Highly Sensitive Alkaline Phosphatase-Based Immunosensors Using 1-Naphthyl Phosphate and an Avidin-Modified Indium Tin Oxide Electrode

被引：6

作者：

Aziz, Md Abdul ^{[1
]}

Jo, Kyungmin ^{[1
]}

Qaium, Md Abdul ^{[1
]}

Huh, Chan-Hwa ^{[1
]}

Hong, In Seok ^{[2
]}

Yang, Haesik ^{[1
]}

机构：

[1] Pusan Natl Univ, Dept Chem, Pusan 609735, South Korea

[2] Kongju Natl Univ, Dept Chem, Kong Ju 314701, South Korea

来源：

ELECTROANALYSIS | 2009年 / 21卷 / 19期

关键词：

Immunosensor; Alkaline phosphatase; 1-Naphthyl phosphate; Avidin; Nonspecific binding; Biosensors; Enzymes; SUPRAMOLECULAR ARCHITECTURES; GOLD NANOPARTICLES; PROTEIN; IMMUNOASSAY; 2-NAPHTHOL; REDUCTION; OXIDATION; BINDING; ASSAY;

D O I：

10.1002/elan.200904641

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

we report a versatile platform for highly sensitive alkaline phosphatase (ALP)-based electrochemical biosensors that uses an avidin-modified indium tin oxide (ITO) electrode as a sensing electrode and 1-naphthyl phosphate (NPP) as an ALP substrate. Almost no electrocatalytic activity of NPP and good electrocatalytic activity of 1-naphthol (ALP product) on the ITO electrodes allow a high signal-to-background ratio. The effective surface covering of avidin on the ITO electrodes allows very low levels of nonspecific binding of proteins to the sensing electrodes. The platform technology is used to detect mouse IgG with a detection limit of 1.0 pg/mL.

引用

页码：2160 / 2164

页数：5

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