The molecular diagnosis of Marteilia refringens and differentiation between Marteilia strains infecting oysters and mussels based on the rDNA IGS sequence

被引：56

作者：

Lopez-Flores, I ^{[1
]}

De La Herrán, R

Garrido-Ramos, MA

Navas, JI

Ruiz-Rejón, C

Ruiz-Rejón, M

机构：

[1] Univ Granada, Fac Ciencias, Dept Genet, Granada, Spain

[2] Consejeria Agr & Pesca, CIFPA Agua Pino, Huelva 21071, Spain

来源：

PARASITOLOGY | 2004年 / 129卷

关键词：

Marteilia refringens; Marteilia maurini; molecular diagnosis; rDNA IGS; Ostrea edulis; Mytilus galloprovincialis;

D O I：

10.1017/S0031182004005827

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Marteilia refringens is a paramyxean parasite which infects the flat oyster Ostrea edulis and mussels (Mytilus galloprovincialis), where it has been attributed to a separate species, Marteilia maurini, by several authors. Doubts persist though as to the existence or not of two species of Marteilia in Europe. We have devised a molecular method for the diagnosis of M. refringens based on 358 bp nested-PCR of the rDNA intergene spacer (rDNA IGS) which is capable of detecting 0(.)5 fg of M. refringens DNA. Molecular characterization of this spacer indicates that the Marteilia parasites which infect oysters and mussels are two different strains of the same species.

引用

页码：411 / 419

页数：9

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