Protein deposition during cross-flow membrane filtration: AFM studies and flux loss

被引：19

作者：

Bowen, WR ^{[1
]}

Doneva, TA ^{[1
]}

Stoton, JAG ^{[1
]}

机构：

[1] Univ Coll Swansea, Dept Chem & Biol Proc Engn, Ctr Complex Fluids Proc, Swansea SA2 8PP, W Glam, Wales

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2003年 / 27卷 / 2-3期

基金：

英国工程与自然科学研究理事会;

关键词：

BSA deposition; membrane filtration; atomic force microscopy;

D O I：

10.1016/S0927-7765(02)00045-0

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

An atomic force microscope (AFM) has been used to study the deposition of bovine serum albumin (BSA) onto the surface of two polymeric membranes of similar molecular weight cut-off but different surface roughness and surface chemistry (ES404 and EM006 PCI membranes (UK)). BSA coated probes were used to quantify the interaction of BSA with both clean and BSA fouled membranes. Clean water flux (CWF) loss suffered by both the membranes under different BSA feed concentrations (0.1, 1 and 10 g l(-1)) and two pHs (pH 5 and 9) was measured in cross-flow filtration experiments. Subsequent comparison was made between the CWF loss, membrane morphology and surface forces for clean and fouled membranes. Protein coverage of membrane surfaces has been determined using profiles of force-distance retraction curves. Preferential adsorption takes place at specific locations on the membrane surface and is a function of pH, surface roughness and surface chemistry. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：103 / 113

页数：11

共 20 条

[1]

BLOOMBERG E, 1991, J DISPERSION SCI TEC, V12, P179

[2] Atomic force microscopy studies of membranes: Effect of surface roughness on double-layer interactions and particle adhesion [J].