Term-seq reveals abundant ribo-regulation of antibiotics resistance in bacteria

被引：249

作者：

Dar, Daniel ^{[1
]}

Shamir, Maya ^{[1
]}

Mellin, J. R. ^{[2
,3
,4
]}

Koutero, Mikael ^{[2
,3
,4
]}

Stern-Ginossar, Noam ^{[1
]}

Cossart, Pascale ^{[2
,3
,4
]}

Sorek, Rotem ^{[1
]}

机构：

[1] Weizmann Inst Sci, Dept Mol Genet, IL-76100 Rehovot, Israel

[2] Inst Pasteur, Unite Interact Bacteries Cellules, F-75015 Paris, France

[3] INSERM, U604, F-75015 Paris, France

[4] INRA, USC2020, F-75015 Paris, France

来源：

SCIENCE | 2016年 / 352卷 / 6282期

基金：

欧洲研究理事会; 以色列科学基金会;

关键词：

CONTROLS GENE-EXPRESSION; MESSENGER-RNA STRUCTURE; BACILLUS-SUBTILIS; LISTERIA-MONOCYTOGENES; ATTENUATION; RIBOSWITCHES; ANTITERMINATION; TRANSCRIPTOMICS; TERMINATION; MECHANISM;

D O I：

10.1126/science.aad9822

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Riboswitches and attenuators are cis-regulatory RNA elements, most of which control bacterial gene expression via metabolite-mediated, premature transcription termination. We developed an unbiased experimental approach for genome-wide discovery of such ribo-regulators in bacteria. We also devised an experimental platform that quantitatively measures the in vivo activity of all such regulators in parallel and enables rapid screening for ribo-regulators that respond to metabolites of choice. Using this approach, we detected numerous antibioticresponsive ribo-regulators that control antibiotic resistance genes in pathogens and in the human microbiome. Studying one such regulator in Listeria monocytogenes revealed an attenuation mechanism mediated by antibiotic-stalled ribosomes. Our results expose broad roles for conditional termination in regulating antibiotic resistance and provide a tool for discovering riboswitches and attenuators that respond to previously unknown ligands.

引用

页数：11

共 58 条

[1] Transcriptional response of Enterococcus faecalis V583 to erythromycin [J].