2,4-Dinitrotoluene (DNT) Perturbs Yolk Absorption, Liver Development and Lipid Metabolism/Oxygen Transport Gene Expression in Zebrafish Embryos and Larvae

被引：8

作者：

Xiong, Jianglin ^{[1
]}

Sha, Hang ^{[2
]}

Zhou, Hualin ^{[3
]}

Peng, Lijuan ^{[4
]}

Wu, Lingying ^{[1
]}

Qiu, Yinsheng ^{[1
]}

Wang, Rui ^{[1
]}

Hu, Xianqin ^{[1
]}

机构：

[1] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China

[2] Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Hubei, Peoples R China

[3] Xiangyang Vocat & Tech Coll, Agr Coll, Xiangyang 441050, Peoples R China

[4] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2019年 / 20卷 / 15期

基金：

中国国家自然科学基金;

关键词：

2; 4-dinitrotoluene; zebrafish (Danio rerio); embryos; liver; yolk; MAMMARY EPITHELIAL-CELLS; TRANSCRIPTIONAL ACTIVATION; HYPOXIA; 2,4,6-TRINITROTOLUENE; TOXICITY; EXPOSURE;

D O I：

10.3390/ijms20153632

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

2,4-dinitrotoluene (2,4-DNT) is a common environmental pollutant, and was classified as a group 2B human carcinogenic compound by the International Agency for Research on Cancer. This study determined the toxic effects of 2,4-DNT exposure on zebrafish at the embryo-larvae stage, in terms of organ morphogenesis and the expression pattern of selected target genes related to lipid metabolism and oxygen transportation. The results showed that the 120-h post-fertilization LC50 of 2,4-DNT was 9.59 mg/L with a 95% confidence interval of 8.89-10.44 mg/L. The larvae treated with 2,4-DNT showed toxic symptoms including smaller body, less skin pigment production, yolk malabsorption, and disordered liver development. Further studies on the expression of genes related to lipid transport and metabolism, and respiration indicated that they were significantly affected by 2,4-DNT. It is concluded that 2,4-DNT exposure perturbed liver development and yolk absorption in early-life zebrafish, and disturbed the lipid metabolism /oxygen transport gene expression.

引用

页数：16

共 39 条

[11]

Lang P.Z., 1998, REACTION TOCIXITY OR

[12] Bioconcentration, elimination and metabolism of 2,4-dinitrotoluene in carps (Cyprinus carpio L.) [J].

Lang, PZ ;

Wang, Y ;

Chen, DB ;

Wang, N ;

Zhao, XM ;

Ding, YZ .

CHEMOSPHERE, 1997, 35 (08) :1799-1815

[13] Hypoxia-inducible factor-1 mediates transcriptional activation of the heme oxygenase-1 gene in response to hypoxia [J].

Lee, PJ ;

Jiang, BH ;

Chin, BY ;

Iyer, NV ;

Alam, J ;

Semenza, GL ;

Choi, AMK .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (09) :5375-5381

[14]

Lent E.M., 2015, WILDLIFE TOXICITY AS

[15] Role of fatty acid binding proteins (FABPs) in cancer development and progression [J].

McKillop, Lain H. ;

Girardi, Cara A. ;

Thompson, Kyle J. .

CELLULAR SIGNALLING, 2019, 62

[16]

Navasa M, 1998, J LIPID RES, V39, P1220

[17] Effects of chronic 2,4,6,-trinitrotoluene, 2,4-dinitrotoluene, and 2,6-dinitrotoluene exposure on developing bullfrog (Rana catesbeiana) tadpoles [J].

Paden, Norka E. ;

Smith, Ernest E. ;

Maul, Jonathan D. ;

Kendall, Ronald J. .

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2011, 74 (04) :924-928

[18]

[庞智勇 Pang Zhiyong], 2011, [环境化学, Environmental Chemistry], V30, P430

[19] Ovarian follicle growth, maturation, and ovulation in teleost fish [J].

Patiño, R ;

Sullivan, CV .

FISH PHYSIOLOGY AND BIOCHEMISTRY, 2002, 26 (01) :57-70

[20] Alterations in lipoprotein metabolism in peroxisome proliferator-activated receptor alpha-deficient mice [J].

Peters, JM ;

Hennuyer, N ;

Staels, B ;

Fruchart, JC ;

Fievet, C ;

Gonzalez, FJ ;

Auwerx, J .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (43) :27307-27312

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