Molecular Characterization of Giardia duodenalis in Children and Adults Sampled in Algeria

被引:9
作者
Belkessa, Salem [1 ,2 ,3 ]
Thomas-Lopez, Daniel [3 ,4 ]
Houali, Karim [1 ]
Ghalmi, Farida [5 ]
Stensvold, Christen Rune [3 ]
机构
[1] Mouloud Mammeri Univ Tizi Ouzou, Lab Analyt Biochem & Biotechnol LABAB, Dept Biochem & Microbiol, Fac Biol & Agron Sci, Tizi Ouzou 15000, Algeria
[2] Mohamed Khider Univ Biskra, Fac Exact Sci & Nat & Life Sci, Dept Nat & Life Sci, Biskra 07000, Algeria
[3] Statens Serum Inst, Parasitol Lab, Dept Bacteria Parasites & Fungi, Infect Dis Preparedness, Artillerivej 5, DK-2300 Copenhagen S, Denmark
[4] European Ctr Dis Prevent & Control ECDC, European Publ Hlth Microbiol Training Programme E, S-16973 Solna, Sweden
[5] Higher Natl Vet Sch Algiers, Algiers 16000, Algeria
关键词
allelic sequence heterozygosity (ASH); diarrhea; Giardia duodenalis; molecular epidemiology; parasite; real-time PCR;
D O I
10.3390/microorganisms9010054
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The molecular epidemiology of giardiasis in Africa remains unclear. A study was carried out across four hospitals in Algeria. A total of 119 fecal samples from 55 children, 37 adults, and 27 individuals of undetermined age, all scored positive for intestinal parasites by microscopy, and were screened by real-time PCR for Giardia. Molecular characterization of Giardia was performed by assemblage-specific PCR and PCR targeting the triose phosphate isomerase gene (tpi). Of the 119 samples, 80 (67%) were Giardia-positive by real-time PCR. For 48 moderately-highly real-time PCR-positive samples, tpi genotyping assigned 22 samples to Assemblage A and 26 to Assemblage B. Contrary to Assemblage A, Assemblage B exhibited substantial genetic diversity and allelic heterozygosity. Assemblage-specific PCR proved to be specific for discriminating Assemblage A or B but not as sensitive as tpi genotyping. We confirmed that real-time PCR is more sensitive than microscopy for detecting Giardia in stool samples and that robust amplification and sequencing of the tpi gene is feasible when moderate-to-strongly real-time PCR-positive samples are used. This study is one of the few performed in Africa providing genotyping data on Giardia infections in humans. Both assemblages A and B were commonly seen and not associated with specific sociodemographic data.
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页码:1 / 11
页数:11
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