Bleach Enhancement of Mixed Wood Pulp by Xylanase-Laccase Concoction Derived Through Co-culture Strategy

被引:50
作者
Dwivedi, Pallavi [1 ]
Vivekanand, V. [1 ]
Pareek, Nidhi [1 ]
Sharma, Amit [1 ]
Singh, Rajesh P. [1 ]
机构
[1] Indian Inst Technol, Dept Biotechnol, Roorkee 247667, Uttar Pradesh, India
关键词
Biobleaching; Penicillium oxalicum; Co-cultivation; Xylanase; Laccase; Pleurotus ostreatus; ENZYME PREPARATIONS; FIBER MORPHOLOGY; WHEAT-STRAW; KRAFT PULP; LIGNIN; DELIGNIFICATION; DEGRADATION; PURIFICATION; PRETREATMENT; OZONE;
D O I
10.1007/s12010-009-8654-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Mixed enzyme preparation having both xylanase and laccase activity was evaluated for its bleach enhancing ability of mixed wood pulp. The enzyme was produced through co-cultivation of mutant Penicillium oxalicum SAU(E)-3.510 and Pleurotus ostreatus MTCC 1804 under solid-state fermentation. Bleaching of pulp with mixed enzyme had resulted into a notable decrease in kappa number and increased brightness as compared to xylanase alone. Analysis of bleaching conditions had denoted that 8 IU g(-1) of mixed enzyme preparation (xylanase/laccase, 22: 1) had led into maximal removal of lignin from pulp when bleaching was performed at 10% pulp consistency (55 degrees C, pH 9.0) for 3 h. An overall improvement of 21%, 8%, 3%, and 5% respectively in kappa number, brightness, yellowness, and viscosity of pulp was achieved under derived bleaching conditions. Process of enzymatic bleaching was further ascertained by analyzing the changes occurring in polysaccharide and lignin by HPLC and FTIR. The UV absorption spectrum of the compounds released during enzymatic treatment had denoted a characteristic peak at 280 nm, indicating the presence of lignin in released coloring matter. The changes in fiber morphology following enzymatic delignification were studied by scanning electron microscopy.
引用
收藏
页码:255 / 268
页数:14
相关论文
共 35 条
[1]   Impact of xylanase pre-treatment on peroxide bleaching stage of biokraft pulp [J].
Atik, Celil ;
Imamoglu, Sami ;
Bermek, Hakan .
INTERNATIONAL BIODETERIORATION & BIODEGRADATION, 2006, 58 (01) :22-26
[2]   BIOBLEACHING OF KRAFT PULP [J].
BAJPAI, P ;
BAJPAI, PK .
PROCESS BIOCHEMISTRY, 1992, 27 (06) :319-325
[3]   Laccase/mediated oxidation of a lignin model for improved delignification procedures [J].
Barreca, AM ;
Fabbrini, M ;
Galli, C ;
Gentili, P ;
Ljunggren, S .
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2003, 26 (1-2) :105-110
[4]   Effect of amino acids on production of xylanase and pectinase from Streptomyces sp QG-11-3 [J].
Beg, QK ;
Bhushan, B ;
Kapoor, M ;
Hoondal, GS .
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2000, 16 (02) :211-213
[5]   FT-IR DETERMINATION OF LIGNIN DEGRADATION IN WHEAT STRAW BY WHITE ROT FUNGUS STROPHARIA-RUGOSOANNULATA WITH DIFFERENT OXYGEN CONCENTRATIONS [J].
BUTA, JG ;
ZADRAZIL, F ;
GALLETTI, GC .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1989, 37 (05) :1382-1384
[6]   Paper pulp delignification using laccase and natural mediators [J].
Camarero, Susana ;
Ibarra, David ;
Martinez, Angel T. ;
Romero, Javier ;
Gutierrez, Ana ;
del Rio, Jos C. .
ENZYME AND MICROBIAL TECHNOLOGY, 2007, 40 (05) :1264-1271
[7]   Extraction and determination of enzymes produced by Ceriporiopsis subvermispora during biopulping of Pinus taeda wood chips [J].
de Souza-Cruz, PB ;
Freer, J ;
Siika-Aho, M ;
Ferraz, A .
ENZYME AND MICROBIAL TECHNOLOGY, 2004, 34 (3-4) :228-234
[8]  
Dutta A., 2017, Spectroscopic Methods for Nanomaterials Characterization, P73, DOI [DOI 10.1016/B978-0-323-46140-5.00004-2, DOI 10.1007/978-3-642-74065-7_16]
[9]   Analytical methods for determining functional groups in various technical lignins [J].
El Mansouri, Nour-Eddine ;
Salvado, Joan .
INDUSTRIAL CROPS AND PRODUCTS, 2007, 26 (02) :116-124
[10]   Bleach boosting effect of cellulase-free xylanase of Streptomyces thermoviolaceus and its comparison with two commercial enzyme preparations on birchwood kraft pulp [J].
Garg, AP ;
Roberts, JC ;
McCarthy, AJ .
ENZYME AND MICROBIAL TECHNOLOGY, 1998, 22 (07) :594-598