Liquid Chromatography Tandem Mass Spectrometry and Nuclear Magnetic Resonance Spectroscopy of Magnesium (II) Gluconate Solution

被引:3
作者
Trivedi, Mahendra Kumar [1 ]
Panda, Parthasarathi [2 ]
Sethi, Kalyan Kumar [2 ]
Jana, Snehasis [2 ]
机构
[1] Trivedi Global Inc, Henderson, NV 89052 USA
[2] Trivedi Sci Res Lab Pvt Ltd, Bhopal 462026, Madhya Pradesh, India
关键词
Magnesium gluconate; Liquid chromatography tandem mass spectrometry; Nuclear magnetic resonance spectroscopy; Organometallic; Electrospray ionization; RATS;
D O I
10.1007/s10953-017-0613-z
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Magnesium gluconate is a classical pharmaceutical compound used as a source of magnesium for the prevention and treatment of hypomagnesemia. To the best of our knowledge, a robust and reliable liquid chromatography tandem mass spectrometry technique has not yet been reported for the qualitative and quantitative analysis of magnesium gluconate. This study describes the method development for the LC-ESI-MS/MS analysis of magnesium gluconate using three different reversed-phase HPLC conditions (Method I-III) with comprehensive fragmentation pattern and the structural characterization by NMR spectroscopy. The LC-MS and NMR data were found in accordance with the structure of magnesium gluconate. When magnesium gluconate was dissolved in the acetonitrile and water-methanol solutions, it exists in situ in three different forms: magnesium gluconate itself, gluconic acid, and magnesium gluconate chelate with gluconic acid by a coordinate covalent bond. Method I exhibited pseudo-molecular ion peaks with more magnesium gluconate chelates with gluconic acid, while method II showed an adduct of magnesium gluconate with the solvent along with the molecular ion peak. There was no pseudo-molecular ion peaks found in method III. Thus, method III was found to be the more accurate, robust and reliable LC-MS method for the qualitative and quantitative analysis, structural characterization, and could also be suitable for the pharmacokinetic study of magnesium gluconate. The detailed fragmentation analysis might be useful for the structural characterization of unknown divalent organometallics.
引用
收藏
页码:896 / 907
页数:12
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