Therapeutic potential of adipose-derived mesenchymal stem cell exosomes in tissue-engineered bladders

被引：17

作者：

Yang, Tianli ^{[1
]}

Zhao, Feng ^{[1
]}

Zhou, Liuhua ^{[1
]}

Liu, Jingyu ^{[1
]}

Xu, Luwei ^{[1
]}

Dou, Quanliang ^{[1
]}

Xu, Zheng ^{[1
]}

Jia, Ruipeng ^{[1
]}

机构：

[1] Nanjing Med Univ, Nanjing Hosp 1, Dept Urol, 68 Changle Rd, Nanjing 210006, Peoples R China

来源：

JOURNAL OF TISSUE ENGINEERING | 2021年 / 12卷

基金：

中国国家自然科学基金;

关键词：

Bladder replacement; tissue engineering; mesenchymal stem cells; exosomes; bladder acellular matrix; angiogenesis;

D O I：

10.1177/20417314211001545

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Mesenchymal stem cells (MSCs) are a therapeutic tool for tissue engineering. However, many studies have recently shown that the therapeutic effects of MSCs are mediated by paracrine signaling and their secretory factors rather than their multidirectional differentiation ability. Exosomes isolated from the conditioned medium of MSCs are considered the main intercellular communication medium between MSCs and their target cells. Exosomes have been utilized in a novel cell-free therapy strategy that has attracted much attention. In this study, we evaluated the effects of a new cell-free tissue-engineered bladder (bladder acellular matrix combined with adipose-derived mesenchymal stem cell exosomes (AMEs)) in vivo and in vitro to prove that AMEs promoted tissue regeneration and functional recovery in a rat bladder replacement model.

引用

页数：14

共 43 条

[1] Comprehensive Proteomic Analysis of Mesenchymal Stem Cell Exosomes Reveals Modulation of Angiogenesis via Nuclear Factor-KappaB Signaling [J].

Anderson, Johnathon D. ;

Johansson, Henrik J. ;

Graham, Calvin S. ;

Vesterlund, Mattias ;

Pham, Missy T. ;

Bramlett, Charles S. ;

Montgomery, Elizabeth N. ;

Mellema, Matt S. ;

Bardini, Renee L. ;

Contreras, Zelenia ;

Hoon, Madeline ;

Bauer, Gerhard ;

Fink, Kyle D. ;

Fury, Brian ;

Hendrix, Kyle J. ;

Chedin, Frederic ;

El-Andaloussi, Samir ;

Hwang, Billie ;

Mulligan, Michael S. ;

Lehtio, Janne ;

Nolta, Jan A. .

STEM CELLS, 2016, 34 (03) :601-613

[2] Extracellular vesicles, exosomes and shedding vesicles in regenerative medicine - a new paradigm for tissue repair [J].

Bjorge, I. M. ;

Kim, S. Y. ;

Mano, J. F. ;

Kalionis, B. ;

Chrzanowski, W. .

BIOMATERIALS SCIENCE, 2017, 6 (01) :60-78

[3] Bladder Augmentation (Enterocystoplasty): the Current State of a Historic Operation [J].

Budzyn, Jeffrey ;

Trinh, Hamilton ;

Raffee, Samantha ;

Atiemo, Humphrey .

CURRENT UROLOGY REPORTS, 2019, 20 (09)

[4] Calnexin Impairs the Antitumor Immunity of CD4+ and CD8+ T Cells [J].

Chen, Yichen ;

Ma, Da ;

Wang, Xi ;

Fang, Juan ;

Liu, Xiangqi ;

Song, Jingjing ;

Li, Xinye ;

Ren, Xianyue ;

Li, Qiusheng ;

Li, Qunxing ;

Wen, Shuqiong ;

Luo, Liqun ;

Xia, Juan ;

Cui, Jun ;

Zeng, Gucheng ;

Chen, Lieping ;

Cheng, Bin ;

Wang, Zhi .

CANCER IMMUNOLOGY RESEARCH, 2019, 7 (01) :123-135

[5] Exosomes from human adipose-derived stem cells promote proliferation and migration of skin fibroblasts [J].

Choi, Eun Wook ;

Seo, Min Koo ;

Woo, Eun Young ;

Kim, Suk Hyung ;

Park, Eun Joo ;

Kim, Sue .

EXPERIMENTAL DERMATOLOGY, 2018, 27 (10) :1170-1172

[6]

Ciuffreda MC, 2016, METHODS MOL BIOL, V1416, P149, DOI 10.1007/978-1-4939-3584-0_8

[7] Participation of the IKK-α/β complex in the inhibition of the TNF-α/NF-kB pathway by glycine: Possible involvement of a membrane receptor specific to adipocytes [J].

Contreras-Nunez, Erika ;

Blancas-Flores, Gerardo ;

Cruz, Miguel ;

Cesar Almanza-Perez, Julio ;

Gomez-Zamudio, Jaime H. ;

Luis Ventura-Gallegosc, Jose ;

Zentella-Dehesa, Alejandro ;

Roberto-Lazzarini ;

Roman-Ramos, Ruben ;

Javier Alarcon-Aguilar, Francisco .

BIOMEDICINE & PHARMACOTHERAPY, 2018, 102 :120-131

[8] PKH26 labeling of extracellular vesicles: Characterization and cellular internalization of contaminating PKH26 nanoparticles [J].

Dominkus, Pia Puzar ;

Stenovec, Matjaz ;

Sitar, Simona ;

Lasic, Eva ;

Zorec, Robert ;

Plemenitas, Ana ;

Zagar, Ema ;

Kreft, Marko ;

Lenassi, Metka .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2018, 1860 (06) :1350-1361

[9] Oral administration of pyrrolidine dithiocarbamate (PDTC) inhibits VEGF expression, tumor angiogenesis and growth of breast cancer in female mice [J].

Gu, Jian-Wei ;

Young, Emily ;

Busby, Brandi ;

Covington, Jordan ;

Tan, Wei ;

Johnson, James W. .

CANCER BIOLOGY & THERAPY, 2009, 8 (06) :514-521

[10] Review of the Isolation, Characterization, Biological Function, and Multifarious Therapeutic Approaches of Exosomes [J].

Gurunathan, Sangiliyandi ;

Kang, Min-Hee ;

Jeyaraj, Muniyandi ;

Qasim, Muhammad ;

Kim, Jin-Hoi .

CELLS, 2019, 8 (04)

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