A three-way junction structure-based isothermal exponential amplification strategy for sensitive detection of 3′-terminal 2′-O-methylated plant microRNA

被引：31

作者：

Wang, Xiangdong ^{[1
]}

Wang, Hui ^{[1
]}

Liu, Chenghui ^{[1
]}

Wang, Honghong ^{[1
]}

Li, Zhengping ^{[1
]}

机构：

[1] Shaanxi Normal Univ, Key Lab Analyt Chem Life Sci Shaanxi Prov, Key Lab Appl Surface & Colloid Chem, Minist Educ,Sch Chem & Chem Engn, Xian 710062, Shaanxi Provinc, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2017年 / 53卷 / 06期

基金：

中国国家自然科学基金;

关键词：

ROLLING-CIRCLE AMPLIFICATION; ARABIDOPSIS-THALIANA; BIOGENESIS; QUANTIFICATION; MIR156; CELLS; STEP; PCR;

D O I：

10.1039/c6cc08726d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Plant microRNAs (miRNAs) differ from animal miRNAs in that they are 2'-O-methylated at the 3' end nucleotide which limits the ability of plant miRNAs to serve as primers for polymerization reactions. Herein, we have rationally designed a three-way junction structure-based isothermal exponential amplification reaction (3WJ-EXPAR) which is well-suited for sensitive detection of 3'-terminal 2'-O-methylated miRNAs.

引用

页码：1124 / 1127

页数：4

共 9 条

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