Surface-modified complex SU-8 microstructures for indirect optical manipulation of single cells

被引：26
作者：
Aekbote, Badri L. [1 ]
Fekete, Tamas [1 ]
Jacak, Jaroslaw [2 ]
Vizsnyiczai, Gaszton [1 ]
Ormos, Pal [1 ]
Kelemen, Lorand [1 ]
机构：
[1] Hungarian Acad Sci, Biol Res Ctr, Temesvari Krt 62, H-6726 Szeged, Hungary
[2] Univ Appl Sci Upper Austria, Garnisonstr 21, A-4020 Linz, Austria
来源：
BIOMEDICAL OPTICS EXPRESS | 2016年 / 7卷 / 01期
关键词：
2-PHOTON POLYMERIZATION; MICROFLUIDIC SYSTEMS; FORCE SPECTROSCOPY; ESCHERICHIA-COLI; TWEEZERS; WAVELENGTH; FABRICATION; ADSORPTION; DEPENDENCE; DAMAGE;
D O I：
10.1364/BOE.7.000045
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We introduce a method that combines two-photon polymerization (TPP) and surface functionalization to enable the indirect optical manipulation of live cells. TPP-made 3D microstructures were coated specifically with a multilayer of the protein streptavidin and non-specifically with IgG antibody using polyethylene glycol diamine as a linker molecule. Protein density on their surfaces was quantified for various coating methods. The streptavidin-coated structures were shown to attach to biotinated cells reproducibly. We performed basic indirect optical micromanipulation tasks with attached structure-cell couples using complex structures and a multi-focus optical trap. The use of such extended manipulators for indirect optical trapping ensures to keep a safe distance between the trapping beams and the sensitive cell and enables their 6 degrees of freedom actuation. (C)2015 Optical Society of America
引用
收藏
页码：45 / 56
页数：12
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