Protease production by thermo-alkaliphilic novel gut isolate Kitasatospora cheerisanensis GAP 12.4 from Gryllotalpa africana

被引:12
作者
Banerjee, Sandipan [1 ]
Maiti, Tushar Kanti [2 ]
Roy, Raj Narayan [1 ]
机构
[1] Dr BN Dutta Smriti Mahavidyalaya, Dept Bot, Microbiol Res Lab, Hatgobindapur 713407, India
[2] Univ Burdwan, Dept Bot, Burdwan, W Bengal, India
关键词
Gryllotalpa; gut; kitasatospora; protease; thermo-alkaliphilic; production; SOLID-STATE FERMENTATION; ALKALINE PROTEASE; BACILLUS-CEREUS; OPTIMIZATION; BACTERIA; CULTURE;
D O I
10.1080/10242422.2017.1306739
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In course of searching for proteolytic microbes from the gut of Gryllotalpa africana, a potent isolate GAP 12.4 was screened and identified as Kitasatospora cheerisanensis having protease activity 46.8 +/- 1.52U/ml. Optimum conditions for the protease production (605.3 +/- 9.7U/ml) were 7-d cultivation, 5% inoculum, pH 9.5, 55 degrees C, 150rpm, and supplementation with 0.8% glucose and 0.6% ammonium sulfate. Surfactants such as SDS, EDTA, Tween 80 and Triton X-100 showed positive effect on enzyme production. Addition of biotin (50g/ml) promotes enzyme production maximally (674.15 +/- 4.13U/ml). Further enhancement on addition of casein hydrolysate and molasses to the production medium was 709.20 +/- 7.53U/ml and 744.26 +/- 9.71U/ml, respectively. The isolate was also able to utilize agro-industries waste, green gram husk in solid-state fermentation for enzyme production (1675.02 +/- 21.58U/ml). This thermo-alkaliphilic isolate may be a potent candidate for low cost protease production through management of agro-residues. It is the first report of protease production by a member of actinobacteria under the Kitasatospora genus.
引用
收藏
页码:168 / 176
页数:9
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