Isopentenyl diphosphate isomerase deficiency in Synechocystis sp strain PCC6803

被引：21

作者：

Ershov, Y ^{[1
]}

Gantt, RR ^{[1
]}

Cunningham, FX ^{[1
]}

Gantt, E ^{[1
]}

机构：

[1] Univ Maryland, Dept Cell Biol & Mol Genet, College Pk, MD 20742 USA

来源：

FEBS LETTERS | 2000年 / 473卷 / 03期

基金：

美国国家科学基金会;

关键词：

dimethylallyl diphosphate; isoprenoid biosynthesis; Escherichia coli; Haematococcus; Synechococcus PCC7942; Synechocystis PCC6803;

D O I：

10.1016/S0014-5793(00)01516-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Isopentenyl diphosphate isomerase (IPP isomerase) in many organisms and in plastids is central to isoprenoid synthesis and involves the conversion between IPP and dimethylallyl diphosphate (DMAPP). It is shown that Synechocystis PCC6803 is deficient in IPP isomerase activity, consistent with the absence in its genome of an obvious homologue for the enzyme. Incorporation of [1-C-14]IPP in cell extracts, primarily into C-20, occurs only upon priming with DMAPP in Synechocystis PCC6803 and in Synechoccus PCC7942. Isoprenoid synthesis in these cyanobacteria does not appear to involve interconversion of IPP and DMAPP, raising the possibility that they are not within the plastid evolutionary lineage. (C) 2000 Federation of European Biochemical Societies.

引用

页码：337 / 340

页数：4

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