LncRNA HOTAIR acts as competing endogenous RNA to control the expression of Notch3 via sponging miR-613 in pancreatic cancer

被引：97

作者：

Cai, Huihua ^{[1
]}

Yao, Jie ^{[2
]}

An, Yong ^{[1
]}

Chen, Xuemin ^{[1
]}

Chen, Weibo ^{[1
]}

Wu, Di ^{[1
]}

Luo, Boyang ^{[1
]}

Yang, Yong ^{[1
]}

Jiang, Yong ^{[1
]}

Sun, Donglin ^{[1
]}

He, Xiaozhou ^{[3
]}

机构：

[1] Soochow Univ, Hosp 3, Peoples Hosp Changzhou 1, Dept Hepatobiliary Surg, Changzhou, Jiangsu, Peoples R China

[2] Yangzhou Univ, Dept Hepatobiliary & Pancreat Surg, Northern Jiangsu Peoples Hosp, Clin Med Coll, Yangzhou, Jiangsu, Peoples R China

[3] Soochow Univ, Hosp 3, Peoples Hosp Changzhou 1, Dept Urol, Changzhou, Jiangsu, Peoples R China

来源：

ONCOTARGET | 2017年 / 8卷 / 20期

关键词：

pancreatic cancer; miR-613; HOTAIR; cell proliferation; invasion and migration; LONG NONCODING RNAS; CELL-PROLIFERATION; PROGNOSTIC BIOMARKER; CLIP-SEQ; INVASION; PROGRESSION; MICRORNAS; INHIBITION; RESISTANCE; MIGRATION;

D O I：

10.18632/oncotarget.16462

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Pancreatic cancer is one of the most deadly cancers with a poor prognosis. Though studies have implicated the roles of microRNAs in pancreatic cancer progression, little is known about the role of miR-613 in pancreatic cancer. In the present study, the expression of miR-613 was down-regulated in pancreatic cancer tissues and cancer cell lines. Down-regulation of miR-613 was positively correlated with tumor differentiation, advanced TNM stage, nodal metastasis and shorter overall survival in patients with pancreatic cancer. Overexpression of miR-613 suppressed cell proliferation, invasion and migration, and induced cell apoptosis and cell cycle arrest at G0/G1 phase in pancreatic cancer cells. Bioinformatics analysis, luciferase reporter assay and rescue experiments showed that notch3 was a direct target of miR-613. MiR-613 was inversely correlated with notch3 expression in pancreatic cancer tissues. The long non-coding RNA, HOX transcript antisense RNA (HOTAIR) was up-regulated in both pancreatic cancer tissues and cancer cell lines, and HOTAIR suppressed the expression of miR-613 via functioning as a competing endogenous RNA. In vivo studies showed that stable overexpression of miR-613 or knock-down of HOTAIR suppressed tumor growth and also reduced the expression of notch3. In conclusion, these results suggest that HOTAIR functions as a competing endogenous RNA to regulate notch3 expression via sponging miR-613 in pancreatic cancer.

引用

页码：32905 / 32917

页数：13

共 39 条

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