Let-7a elevates p21WAF1 levels by targeting of NIRF and suppresses the growth of A549 lung cancer cells

被引：72

作者：

He, Xiaoyan ^{[1
,2
]}

Duan, Changzhu ^{[1
,2
]}

Chen, Junxia ^{[1
,2
]}

Ou-Yang, Xi ^{[1
]}

Zhang, Zheng ^{[1
]}

Li, Chunlei ^{[1
]}

Peng, Huimin ^{[1
,2
]}

机构：

[1] Chongqing Med Univ, Dept Mol Genet & Cell Biol, Chongqing 400016, Peoples R China

[2] Chongqing Med Univ, Mol Med & Canc Res Ctr, Chongqing 400016, Peoples R China

来源：

FEBS LETTERS | 2009年 / 583卷 / 21期

基金：

中国国家自然科学基金;

关键词：

MicroRNA; Let-7a; NIRF; p21(WAF1); Lung cancer; GENOMIC HYBRIDIZATION ANALYSIS; MICRORNA; EXPRESSION; REPRESSES; ONCOGENE; SURVIVAL; SEQUENCE; PROTEIN; MIRNAS; FAMILY;

D O I：

10.1016/j.febslet.2009.10.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Down-regulation of let-7 microRNA (miRNA) is a key event in lung cancer. Despite recent advances in survival signaling, the roles of let-7 in the context of lung cancer are not fully clear. In this study, we showed that let-7a, a member of let-7 family, negatively regulated the expression of NIRF through NIRF 3' UTR. We also showed that NIRF was required for the let-7a-mediated elevation of p21(WAF1). These findings suggest that growth-inhibitory effect of let-7a on the A549 cells in vitro and in vivo may be explained in part by le-7a-induced suppression of NIRF and elevation of p21(WAF1). This work reveals a novel regulatory mechanism for let-7a in the control of cellular proliferation and lung carcinogenesis. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：3501 / 3507

页数：7

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