Rapid expression of Mycobacterium avium subsp paratuberculosis recombinant proteins for antigen discovery

被引：20
作者：
Li, Lingling
Munir, Shirin
Bannantine, John P.
Sreevatsan, Srinand
Kanjilal, Sagarika
Kapur, Vivek
机构：
[1] Univ Minnesota, Dept Microbiol, St Paul, MN 55108 USA
[2] Univ Minnesota, Biomed Genom Ctr, St Paul, MN 55108 USA
[3] Univ Minnesota, Dept Vet Populat Med, St Paul, MN 55108 USA
[4] Univ Minnesota, Dept Med, St Paul, MN 55108 USA
[5] USDA ARS, Natl Anim Dis Ctr, Ames, IA 50010 USA
来源：
CLINICAL AND VACCINE IMMUNOLOGY | 2007年 / 14卷 / 01期
关键词：
D O I：
10.1128/CVI.00138-06
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Mycobacterium avium subsp. paratuberculosis is the causative agent of Johne's disease, a chronic granulomatous enteritis of ruminants and other species. Detection of infection in animals is hampered by the lack of sensitive and specific diagnostic assays. We describe here an approach that utilizes translationally active PCR fragments for the rapid in vitro transcription and translation of recombinant proteins for antigen discovery in M. avium subsp. paratuberculosis. The investigations showed that the MAP1272c protein selectively reacts with sera from Johne's disease-positive cattle and represents an antigen of potential utility in M. avium subsp. paratuberculosis immunodiagnostics.
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收藏
页码：102 / 105
页数：4
相关论文
共 14 条
[1]   Predictive estimation of protein linear epitopes by using the program PEOPLE [J].
Alix, AJP .
VACCINE, 1999, 18 (3-4) :311-314
[2]   Expression and immunogenicity of proteins encoded by sequences specific to Mycobacterium avium subsp paratuberculosis [J].
Bannantine, JP ;
Hansen, JK ;
Paustian, ML ;
Amonsin, A ;
Li, LL ;
Stabel, JR ;
Kapur, V .
JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (01) :106-114
[3]   The Mycobacterium avium subsp paratuberculosis 35 kDa protein plays a role in invasion of bovine epithelial cells [J].
Bannantine, JP ;
Huntley, JFJ ;
Miltner, E ;
Stabel, JR ;
Bermudez, LE .
MICROBIOLOGY-SGM, 2003, 149 :2061-2069
[4]   Genomic homogeneity between Mycobacterium avium subsp avium and Mycobacterium avium subsp paratuberculosis belies their divergent growth rates -: art. no. 10 [J].
Bannantine, JP ;
Zhang, Q ;
Li, LL ;
Kapur, V .
BMC MICROBIOLOGY, 2003, 3 (1) :1-10
[5]   Johne's disease, inflammatory bowel disease, and Mycobacterium paratuberculosis [J].
Chacon, O ;
Bermudez, LE ;
Barletta, RG .
ANNUAL REVIEW OF MICROBIOLOGY, 2004, 58 :329-363
[6]   Evaluation of five antibody detection tests for diagnosis of bovine paratuberculosis [J].
Collins, MT ;
Wells, SJ ;
Petrini, KR ;
Collins, JE ;
Schultz, RD ;
Whitlock, RH .
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 2005, 12 (06) :685-692
[7]   COMPARISON OF A COMPLEMENT-FIXATION TEST, A GEL-DIFFUSION TEST AND 2 ABSORBED AND UNABSORBED ELISAS FOR THE DIAGNOSIS OF PARATUBERCULOSIS IN SHEEP [J].
HILBINK, F ;
WEST, DM ;
DELISLE, GW ;
KITTELBERGER, R ;
HOSIE, BD ;
HUTTON, J ;
COOKE, MM ;
PENROSE, M .
VETERINARY MICROBIOLOGY, 1994, 41 (1-2) :107-116
[8]   CEP: a conformational epitope prediction server [J].
Kulkarni-Kale, U ;
Bhosle, S ;
Kolaskar, AS .
NUCLEIC ACIDS RESEARCH, 2005, 33 :W168-W171
[9]   Determination of a 15437 bp nucleotide sequence around the inhA gene of Mycobacterium avium and similarity analysis of the products of putative ORFs [J].
Labò, M ;
Gusberti, L ;
De Rossi, E ;
Speziale, P ;
Riccardi, G .
MICROBIOLOGY-UK, 1998, 144 :807-814
[10]   The complete genome sequence of Mycobacterium avium subspecies paratuberculosis [J].
Li, LL ;
Bannantine, JP ;
Zhang, Q ;
Amonsin, A ;
May, BJ ;
Alt, D ;
Banerji, N ;
Kanjilal, S ;
Kapur, V .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (35) :12344-12349
12