Potential applications of microfluidics based blood brain barrier (BBB)-on-chips for in vitro drug development

被引：39

作者：

Wang, Xiaobo ^{[1
,2
]}

Hou, Ya ^{[1
,2
]}

Ai, Xiaopeng ^{[2
]}

Sun, Jiayi ^{[1
]}

Xu, Binjie ^{[1
]}

Meng, Xianli ^{[1
,2
]}

Zhang, Yi ^{[2
,3
]}

Zhang, Sanyin ^{[1
]}

机构：

[1] Chengdu Univ Tradit Chinese Med, Innovat Inst Chinese Med & Pharm, Chengdu 611137, Peoples R China

[2] Chengdu Univ Tradit Chinese Med, Ethn Med Acad Heritage Innovat Res Ctr, Chengdu 611137, Peoples R China

[3] Chengdu Univ Tradit Chinese Med, NMPA Key Lab Qual Evaluat Tradit Chinese Med Trad, Chengdu 611137, Peoples R China

来源：

BIOMEDICINE & PHARMACOTHERAPY | 2020年 / 132卷

基金：

中国国家自然科学基金; 国家重点研发计划;

关键词：

Microfluidics; BBB-on-chips; Screening active ingredients; Effectiveness evaluation; ORGANS-ON-CHIPS; DISEASE; MODEL; FABRICATION; MICROBIOTA; ARRAY;

D O I：

10.1016/j.biopha.2020.110822

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The human blood-brain barrier (BBB) is a complex multi-dimensional reticular barrier system composed of cerebral microvascular endothelial cells, pericytes, astrocytes and a variety of neurons. The conventional in vitro cell culture model fails to truly present the dynamic hemodynamics of BBB and the interaction between neurons. And it is even more impossible to explore brain-related multi-organ diseases, which brings huge obstacles to explore diseases of the central nervous system and the interaction between brain-related multi-organs, and evaluate drug efficacy. Miniaturized microfluidics based BBB chips are being commonly used to co-culture a variety of cells on a small-sized chip to construct a three-dimensional (3D) BBB or BBB-related organ disease models. By combining with other electrophysiological, biochemical sensors or equipment and imaging systems, it can in real time and quickly screen disease-related markers and evaluate drug efficacy. This review systematically summarized the research progress of in vitro BBB and BBB-related organ chips, and analyzed the obstacles of BBB models in depth. Parallelly combined with the current research trends and hot spots, we give the further improvement measures of microfluidic BBB chips.

引用

页数：7

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