Optimization of Culture Conditions for the Biodegradation of Lindane by the Polypore Fungus Ganoderma australe

被引:40
作者
Dritsa, V. [1 ]
Rigas, F. [1 ]
Doulia, D. [1 ]
Avramides, E. J. [2 ]
Hatzianestis, I. [3 ]
机构
[1] Natl Tech Univ Athens, Sch Chem Engn, Athens 15700, Greece
[2] Natl Agr Res Fdn, Athens Soil Sci Inst, Athens, Greece
[3] Natl Ctr Marine Res, GR-16604 Athens, Greece
关键词
Biodegradation; Lindane; Central composite design (CCD); Response surface methodology (RSM); Ganoderma australe; WHITE-ROT FUNGI;
D O I
10.1007/s11270-009-0022-z
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The bracket-like polypore fungus, Ganoderma australe, was selected for its potential to degrade lindane in liquid agitated sterile cultures. An orthogonal central composite design based on response surface methodology was used to find the optimum biodegradation and biosorption conditions of this pesticide and the growth conditions of the fungus. The factors tested include nitrogen content, initial concentration of lindane, incubation time, and temperature. The optimization parameters investigated were fungus biomass, fungus growth rate, final pH, specific biodegradation, specific biosorption, specific biodegradation rate, biodegraded to biosorbed ratio. The results of the experiments were statistically analyzed and the significance and effect of each factor on responses was assessed. The optimum (maximum) lindane biodegradation (3.11 mg biodegraded lindane per gram biomass) was obtained with nitrogen content of 1.28 g/L, lindane concentration of 7.0 ppm, temperature of 18.0A degrees C, and 5 days of cultivation time.
引用
收藏
页码:19 / 27
页数:9
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