Microautoradiography of L-[3H]DOPA uptake sites in the rat brain

被引：0

作者：

Sugaya, Y ^{[1
]}

Sasaki, Y ^{[1
]}

Goshima, Y ^{[1
]}

Kusakabe, T ^{[1
]}

Kato, T ^{[1
]}

Misu, Y ^{[1
]}

机构：

[1] Yokohama City Univ, Sch Med, Dept Pharmacol, Yokohama, Kanagawa, Japan

来源：

FRONTIERS OF THE MECHANISMS OF MEMORY AND DEMENTIA | 2000年 / 1200期

关键词：

neurotransmitter; transporter; Na+-dependent uptake; distribution;

D O I：

暂无

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We have proposed that L-3,4-dihydroxyphenylalanine (L-DOPA) is a neurotransmitter in the central nervous system. We examined L-DOPA uptake sites in rat brain slices using radiolabelled L-DOPA In the autoradiography with L-[C-14]-DOPA, the uptake activities were seen in cerebral cortex, hypothalamus and hippocampus, and these labeling were inhibited by cold L-DOPA or L-phenylalanine, but not by dopamine. In microautoradiography with L-[H-3]DOPA, we found that a high silver grain density in the hypothalamus, hippocampus, medulla oblongata and cerebellum, but low in the striatum. Under Nat-free condition, density of grains in hippocampus was significantly reduced. These data suggest that there exist some neural cells which uptake L-DOPA in a Na+-dependent manner in the CNS.

引用

页码：41 / 42

页数：2