Cell-free protein synthesis of norovirus virus-like particles

被引：22

作者：

Sheng, Jiayuan ^{[1
]}

Lei, Shaohua ^{[2
]}

Yuan, Lijuan ^{[2
]}

Feng, Xueyang ^{[1
]}

机构：

[1] Virginia Polytech Inst & State Univ, Dept Biol Syst Engn, Blacksburg, VA 24061 USA

[2] Virginia Polytech Inst & State Univ, Dept Biomed Sci & Pathobiol, Blacksburg, VA 24061 USA

来源：

RSC ADVANCES | 2017年 / 7卷 / 46期

关键词：

FREE SYSTEM; EXPRESSION; STABILITY; PH;

D O I：

10.1039/c7ra03742b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Norovirus vaccine development largely depends on recombinant virus-like-particles (VLPs). Norovirus VLPs have been produced in several cell-based expression systems with long production times. Here we report, for the first time, that norovirus VLPs can be expressed and assembled by using a cell-free protein expression system within four hours.

引用

页码：28837 / 28840

页数：4

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