Genetically engineered avidins and streptavidins

被引：272
作者：
Laitinen, O. H.
Hytoenen, V. P.
Nordlund, H. R.
Kulomaa, M. S.
机构：
[1] Univ Tampere, Inst Med Technol, Tampere 33014, Finland
[2] Univ Kuopio, Dept Mol Med, AI Virtanen Inst, FIN-70211 Kuopio, Finland
[3] ETH, Dept Mat, CH-8093 Zurich, Switzerland
来源：
CELLULAR AND MOLECULAR LIFE SCIENCES | 2006年 / 63卷 / 24期
关键词：
affinity; avidin; bionanotechnology; biotin; mutagenesis; protein engineering; streptavidin;
D O I：
10.1007/s00018-006-6288-z
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Chicken avidin and bacterial streptavidin, (strept)avidin, are proteins widely utilized in a number of applications in life science, ranging from purification and labeling techniques to diagnostics, and from targeted drug delivery to nanotechnology. (Strept)avidin-biotin technology relies on the extremely tight and specific affinity between (strept)avidin and biotin (dissociation constant, Kd approximate to 10(-14)-10(-16) M). (Strept)avidins are also exceptionally stable proteins. To study their ligand binding and stability characteristics, the two proteins have been extensively modified both chemically and genetically. There are excellent accounts of this technology and chemically modified (strept)avidins, but no comprehensive reviews exist concerning genetically engineered (strept)avidins. To fill this gap, we here go through the genetically engineered (strept)avidins, summarizing how these constructs were designed and how they have improved our understanding of the structural and functional characteristics of these proteins, and the benefits they have provided for (strept)avidin-biotin technology.
引用
收藏
页码：2992 / 3017
页数：26
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