Calcium EXAFS establishes the Mn-Ca cluster in the oxygen-evolving complex of photosystem II

被引：120
作者：
Cinco, RM
Holman, KLM
Robblee, JH
Yano, J
Pizarro, SA
Bellacchio, E
Sauer, K
Yachandra, VK [1 ]
机构：
[1] Lawrence Berkeley Natl Lab, Melvin Calvin Lab, Phys Biosci Div, Berkeley, CA 94720 USA
[2] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA
来源：
BIOCHEMISTRY | 2002年 / 41卷 / 43期
关键词：
D O I：
10.1021/bi026569p
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The proximity of Ca to the Mn cluster of the photosynthetic water-oxidation complex is, demonstrated by X-ray absorption spectroscopy. We have collected EXAFS data at the Ca K-edge using active PS 11 membrane samples that contain approximately 2 Ca per 4 Mn. These samples are much less perturbed than previously investigated Sr-substituted samples, which were prepared after Ca depletion. The new Ca EXAFS clearly shows backscattering from Mn at 3.4 Angstrom, a distance that agrees with that surmised from previously recorded Mn EXAFS. This result is also consistent with earlier related experiments at the Sr K-edge, using samples that contained functional Sr, that show Mn is similar to3.5 Angstrom distant from Sr. The totality of the evidence clearly advances the notion that the catalytic center of oxygen evolution is a Mn-Ca heteronuclear cluster.
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页码：12928 / 12933
页数：6
相关论文
共 62 条
[1]   STUDIES OF CA2+ BINDING IN SPINACH PHOTOSYSTEM-II USING CA-45(2+) [J].
ADELROTH, P ;
LINDBERG, K ;
ANDREASSON, LE .
BIOCHEMISTRY, 1995, 34 (28) :9021-9027
[2]   Assembly of the tetra-Mn site of photosynthetic water oxidation by photoactivation: Mn stoichiometry and detection of a new intermediate [J].
Ananyev, GM ;
Dismukes, GC .
BIOCHEMISTRY, 1996, 35 (13) :4102-4109
[3]   High-resolution kinetic studies of the reassembly of the tetra-manganese cluster of photosynthetic water oxidation: Proton equilibrium, cations, and electrostatics [J].
Ananyev, GM ;
Dismukes, GC .
BIOCHEMISTRY, 1996, 35 (46) :14608-14617
[4]   Calcium induces binding and formation of a spin-coupled dimanganese(II,II) center in the apo-water oxidation complex of photosystem II as precursor to the functional tetra-Mn/Ca cluster [J].
Ananyev, GM ;
Dismukes, GC .
BIOCHEMISTRY, 1997, 36 (38) :11342-11350
[5]   A HIGHLY RESOLVED, OXYGEN-EVOLVING PHOTOSYSTEM-II PREPARATION FROM SPINACH THYLAKOID MEMBRANES - ELECTRON-PARAMAGNETIC-RES AND ELECTRON-TRANSPORT PROPERTIES [J].
BERTHOLD, DA ;
BABCOCK, GT ;
YOCUM, CF .
FEBS LETTERS, 1981, 134 (02) :231-234
[6]   X-RAY ABSORPTION NEAR EDGE STRUCTURE (XANES) DETERMINATION OF CALCIUM SITES OF TROPONIN-C AND PARVALBUMIN [J].
BIANCONI, A ;
GIOVANNELLI, A ;
CASTELLANI, L ;
ALEMA, S ;
FASELLA, P ;
OESCH, B ;
MOBILIO, S .
JOURNAL OF MOLECULAR BIOLOGY, 1983, 165 (01) :125-138
[7]   Location of the calcium binding site in Photosystem II: A Mn2+ substitution study [J].
Booth, PJ ;
Rutherford, AW ;
Boussac, A .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1996, 1277 (1-2) :127-134
[8]  
BOUSSAC A, 1988, CHEM SCRIPTA, V28A, P123
[9]   THE OXYGEN-EVOLVING ENZYME - EFFECTS OF CALCIUM AND CHLORIDE-IONS [J].
BOUSSAC, A ;
RUTHERFORD, AW .
BIOCHEMICAL SOCIETY TRANSACTIONS, 1994, 22 (02) :352-358
[10]   NATURE OF THE INHIBITION OF THE OXYGEN-EVOLVING ENZYME OF PHOTOSYSTEM-II INDUCED BY NACL WASHING AND REVERSED BY THE ADDITION OF CA-2+ OR SR-2+ [J].
BOUSSAC, A ;
RUTHERFORD, AW .
BIOCHEMISTRY, 1988, 27 (09) :3476-3483
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