The Effect of 100 mT SMF on Activation of the hsp70 Promoter in a Heat Shock/Luciferase Reporter System

被引:3
|
作者
Belton, Michelle [1 ]
Rozanski, Camilla [1 ,2 ]
Prato, Frank S. [1 ,2 ]
Carson, Jeffrey J. L. [1 ,2 ]
机构
[1] Lawson Hlth Res Inst, Imaging Program, London, ON N6A 4V2, Canada
[2] Univ Western Ontario, Dept Med Biophys, London, ON, Canada
基金
加拿大健康研究院;
关键词
REAL-TIME; HEAT SHOCK; BIOLUMINESCENCE; STRESS RESPONSE; CYTOSOLIC-FREE CALCIUM; STATIC MAGNETIC-FIELD; HZ ELECTROMAGNETIC-FIELDS; REAL-TIME MEASUREMENT; STRESS-PROTEINS; MESSENGER-RNA; HL-60; CELLS; EXPOSURE; EXPRESSION; HEAT-SHOCK-PROTEIN-70;
D O I
10.1002/jcb.22327
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Human exposure to magnetic fields, increased through use of new technologies like magnetic resonance imaging (MRI), has prompted investigations into possible effects of static magnetic fields (SMFs) on cellular processes. However, controversy still remains between many studies, which likely results from a lack of uniformity across experimental parameters, including the length of magnetic field exposure, the strength of the magnetic field, and the cell type or organism under investigation. The purpose of this research was to monitor effects of SMF exposure using real-time luminescence photometry. The study investigated the potential interaction of a 100 mT SMF on a heat shock protein (hsp70)/luciferase reporter construct in stably transfected NIH3T3 cells. Changes in heat shock promoter activation following 100 mT SMF exposure were analyzed and detected as bioluminescence in real-time. Two heat parameters were considered in combination with sham- and 100 mT-exposed experiments: no heat or 1,800 s heat. As expected, there was a significant increase in bioluminescence in response to 1,800 s of heat alone. However, no significant difference in average hsp70 promoter activation between sham and 100 mT experiments was observed for no heat or 1,800 s heat experiments. Therefore, a 100 mT SMF was shown to have no effect on the activation of the heat shock protein promoter during SMF exposure or when SMF exposure was combined with a heat insult. J. Cell. Biochem. 108: 956-962, 2009. (C) 2009 Wiley-Liss, Inc.
引用
收藏
页码:956 / 962
页数:7
相关论文
共 50 条
  • [1] Effect of Glutathione Depletion, Hyperthermia, and a 100-mT Static Magnetic Field on an hsp70/luc Reporter System
    Belton, Michelle
    Prato, Frank S.
    Carson, Jeffrey J. L.
    BIOELECTROMAGNETICS, 2011, 32 (06) : 453 - 462
  • [2] An Hsp70 promoter-based mouse for heat shock-induced gene modulation
    Chen, Hangang
    Xie, Yangli
    Zhang, Mei
    Huang, Junlan
    Jiang, Wanling
    Zhang, Ruobin
    Li, Can
    Du, Xiaolan
    Chen, Hua
    Nie, Qiang
    Liang, Sen
    Tan, Qiaoyan
    Yang, Jing
    Jin, Min
    Huang, Shuo
    Kuang, Liang
    Su, Nan
    Qi, Huabing
    Luo, Xiaoqing
    Xu, Xiaoling
    Deng, Chuxia
    Chen, Lin
    Luo, Fengtao
    JOURNAL OF MOLECULAR MEDICINE-JMM, 2024, 102 (05): : 693 - 707
  • [3] An Hsp70 promoter–based mouse for heat shock–induced gene modulation
    Hangang Chen
    Yangli Xie
    Mei Zhang
    Junlan Huang
    Wanling Jiang
    Ruobin Zhang
    Can Li
    Xiaolan Du
    Hua Chen
    Qiang Nie
    Sen Liang
    Qiaoyan Tan
    Jing Yang
    Min Jin
    Shuo Huang
    Liang Kuang
    Nan Su
    Huabing Qi
    Xiaoqing Luo
    Xiaoling Xu
    Chuxia Deng
    Lin Chen
    Fengtao Luo
    Journal of Molecular Medicine, 2024, 102 : 693 - 707
  • [4] HSP70 is essential to the neuroprotective effect of heat-shock
    Sato, K
    Saito, H
    Matsuki, N
    BRAIN RESEARCH, 1996, 740 (1-2) : 117 - 123
  • [5] Compound A, a Selective Glucocorticoid Receptor Modulator, Enhances Heat Shock Protein Hsp70 Gene Promoter Activation
    Beck, Ilse M.
    Drebert, Zuzanna J.
    Hoya-Arias, Ruben
    Bahar, Ali A.
    Devos, Michael
    Clarisse, Dorien
    Desmet, Sofie
    Bougarne, Nadia
    Ruttens, Bart
    Gossye, Valerie
    Denecker, Geertrui
    Lievens, Sam
    Bracke, Marc
    Tavernier, Jan
    Declercq, Wim
    Gevaert, Kris
    Vanden Berghe, Wim
    Haegeman, Guy
    De Bosscher, Karolien
    PLOS ONE, 2013, 8 (07):
  • [6] cDNA of canine heat shock protein 70 (HSP70)
    Kano, R
    Abe, K
    Hasegawa, A
    VETERINARY RESEARCH COMMUNICATIONS, 2004, 28 (05) : 395 - 405
  • [7] cDNA of Canine Heat Shock Protein 70 (HSP70)
    R. Kano
    K. Abe
    A. Hasegawa
    Veterinary Research Communications, 2004, 28 : 395 - 405
  • [8] Hsp70 dynamics in vivo: effect of heat shock and protein aggregation
    Zeng, XC
    Bhasin, S
    Wu, XF
    Lee, JG
    Maffi, S
    Nichols, CJ
    Lee, KJ
    Taylor, JP
    Greene, LE
    Eisenberg, E
    JOURNAL OF CELL SCIENCE, 2004, 117 (21) : 4991 - 5000
  • [9] Membrane-anchored heat-shock protein 70 (Hsp70) in cancer
    Elmallah, Mohammed I. Y.
    Cordonnier, Marine
    Vautrot, Valentin
    Chanteloup, Gaetan
    Garrido, Carmen
    Gobbo, Jessica
    CANCER LETTERS, 2020, 469 : 134 - 141
  • [10] HSP70 Transgene Directed Motion to Nuclear Speckles Facilitates Heat Shock Activation
    Khanna, Nimish
    Hu, Yan
    Belmont, Andrew S.
    CURRENT BIOLOGY, 2014, 24 (10) : 1138 - 1144