The Effect of 100 mT SMF on Activation of the hsp70 Promoter in a Heat Shock/Luciferase Reporter System

被引:3
|
作者
Belton, Michelle [1 ]
Rozanski, Camilla [1 ,2 ]
Prato, Frank S. [1 ,2 ]
Carson, Jeffrey J. L. [1 ,2 ]
机构
[1] Lawson Hlth Res Inst, Imaging Program, London, ON N6A 4V2, Canada
[2] Univ Western Ontario, Dept Med Biophys, London, ON, Canada
基金
加拿大健康研究院;
关键词
REAL-TIME; HEAT SHOCK; BIOLUMINESCENCE; STRESS RESPONSE; CYTOSOLIC-FREE CALCIUM; STATIC MAGNETIC-FIELD; HZ ELECTROMAGNETIC-FIELDS; REAL-TIME MEASUREMENT; STRESS-PROTEINS; MESSENGER-RNA; HL-60; CELLS; EXPOSURE; EXPRESSION; HEAT-SHOCK-PROTEIN-70;
D O I
10.1002/jcb.22327
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Human exposure to magnetic fields, increased through use of new technologies like magnetic resonance imaging (MRI), has prompted investigations into possible effects of static magnetic fields (SMFs) on cellular processes. However, controversy still remains between many studies, which likely results from a lack of uniformity across experimental parameters, including the length of magnetic field exposure, the strength of the magnetic field, and the cell type or organism under investigation. The purpose of this research was to monitor effects of SMF exposure using real-time luminescence photometry. The study investigated the potential interaction of a 100 mT SMF on a heat shock protein (hsp70)/luciferase reporter construct in stably transfected NIH3T3 cells. Changes in heat shock promoter activation following 100 mT SMF exposure were analyzed and detected as bioluminescence in real-time. Two heat parameters were considered in combination with sham- and 100 mT-exposed experiments: no heat or 1,800 s heat. As expected, there was a significant increase in bioluminescence in response to 1,800 s of heat alone. However, no significant difference in average hsp70 promoter activation between sham and 100 mT experiments was observed for no heat or 1,800 s heat experiments. Therefore, a 100 mT SMF was shown to have no effect on the activation of the heat shock protein promoter during SMF exposure or when SMF exposure was combined with a heat insult. J. Cell. Biochem. 108: 956-962, 2009. (C) 2009 Wiley-Liss, Inc.
引用
收藏
页码:956 / 962
页数:7
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