Development of a Multiplex Real-Time PCR for Detection and Differentiation of Malignant Catarrhal Fever Viruses in Clinical Samples

被引：35

作者：

Cunha, Cristina W. ^{[1
]}

Otto, Lisa ^{[2
]}

Taus, Naomi S. ^{[1
]}

Knowles, Donald P. ^{[1
,2
]}

Li, Hong ^{[1
]}

机构：

[1] Washington State Univ, Anim Dis Res Unit, ARS, USDA, Pullman, WA 99164 USA

[2] Washington State Univ, Coll Vet Med, Pullman, WA 99164 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2009年 / 47卷 / 08期

关键词：

DEER ODOCOILEUS-VIRGINIANUS; BISON BISON-BISON; OVINE HERPESVIRUS-2; SIKA-DEER; SHEEP; WILDEBEEST; INFECTION; DIAGNOSIS; FEEDLOT; OVHV-2;

D O I：

10.1128/JCM.00997-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A multiplex real-time PCR was developed using a single pair of primers and fluorescent probes specific for five malignant catarrhal fever viruses and an internal positive control. The assay was able to simultaneously detect and differentiate the viruses in clinical samples with high sensitivity (97.2%) and specificity (100%).

引用

页码：2586 / 2589

页数：4

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