Molecular cloning and expression analysis of pig lymphocyte activation gene-3 (LAG-3; CD223)

被引：11

作者：

Kim, Seon-Soo ^{[1
,2
]}

Kim, Sang-Hoon ^{[1
]}

Kang, Hyung-Sik ^{[3
]}

Chung, Hee Yong ^{[2
]}

Choi, Inho ^{[4
]}

Cheon, Yong-Pil ^{[5
]}

Lee, Ki Ho ^{[6
]}

Lee, Dong-Mok ^{[4
]}

Park, Jongsun ^{[7
]}

Lee, Sang Yeol ^{[8
]}

Chun, Taehoon ^{[1
]}

机构：

[1] Korea Univ, Sch Life Sci & Biotechnol, Seoul 136701, South Korea

[2] Hanyang Univ, Dept Microbiol & Immunol, Sch Med, Seoul 133791, South Korea

[3] Chonnam Natl Univ, Sch Biol Sci & Technol, Kwangju 500757, South Korea

[4] Yeungnam Univ, Sch Biotechnol, Gyongsan 712749, South Korea

[5] Sungshin Womens Univ, Coll Nat Sci, Dept Biol, Inst Basic Sci, Seoul 136742, South Korea

[6] Eulji Univ, Sch Med, Dept Biochem & Mol Biol, Taejon 301832, South Korea

[7] Chungnam Natl Univ, Cell Signaling Lab, Canc Res Inst, Res Inst Med Sci,Coll Med, Taejon 305764, South Korea

[8] Kyungwon Univ, Coll Nat Sci, Dept Life Sci, Seoul 461701, South Korea

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 2010年 / 133卷 / 01期

关键词：

Co-receptor; Immune homeostasis; Lymphocyte activation gene-3; Pig; T cell activation; T-CELLS; PROTEIN; PROLIFERATION; ANTIGENS; DNA;

D O I：

10.1016/j.vetimm.2009.07.001

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Lymphocyte activation gene-3 (LAG-3; CD223) is a co-receptor that may function as a down-modulator of T cell activity via an interaction with MHC class II molecules. In this study, we cloned and sequenced the complete cDNA sequence of pig Lag3. Pig Lag3 cDNA contains an open reading frame (1524 bp) encoding 507 amino acids. The putative amino acid identity of pig LAG-3 with those of the human, rat, and mouse is 78%, 66%, and 67%, respectively. Pig Lag3 mRNA transcript was mainly expressed in lymphoid tissues, and was inducible upon stimulation of phytohemagglutinin (PHA). Next, we developed mouse antiserum against the extracellular domain (D1 and D2 domains) of pig LAG-3. Pig LAG-3 is detected as an approximately 67 kDa of a single species by Western blot analysis. Moreover, flow cytometry analysis confirmed that LAG-3 on pig T cells (CD3(+) cells) is inducible upon PHA stimulation. These results indicated that the structure and expression pattern of pig LAG-3 are conserved among mammalian species. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：72 / 79

页数：8

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