Genome mining of cryptic tetronate natural products from a PKS-NRPS encoding gene cluster in Trichoderma harzianum t-22

被引：2

作者：

Zhu, Yiguang ^{[1
,2
]}

Wang, Junfeng ^{[1
,2
]}

Mou, Pengyun ^{[2
]}

Yan, Yan ^{[1
,2
]}

Chen, Mengbin ^{[1
]}

Tang, Yi ^{[1
]}

机构：

[1] Univ Calif Los Angeles, Dept Chem & Biomol Engn, Los Angeles, CA 90095 USA

[2] Chinese Acad Sci, Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med, South China Sea Inst Oceanol, Guangzhou 510301, Peoples R China

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2021年 / 19卷 / 09期

基金：

美国国家科学基金会;

关键词：

Carboxylation - Bacteria - Ketones - Biocontrol - Encoding (symbols) - Gene encoding - Signal encoding;

D O I：

10.1039/d0ob02545c

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Trichoderma harzianum is a widely used biocontrol agent in agriculture. Obtaining a full inventory of the small molecules that can be biosynthesized from the encoded biosynthetic gene clusters (BGCs) is therefore useful for understanding associated plant-microbe and microbe-microbe interactions. Here we heterologously reconstituted a polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) encoding gene cluster from T. harzianum t-22 in Aspergillus nidulans A1145. Six new tetronate natural products trihazone A-F (1-6) were isolated and elucidated by HRESIMS and 1D and 2D NMR data. Three of the products contain an exocyclic olefin, which is derived from the oxidative decarboxylation of an alpha-ketoglutarate-dependent dioxygenase ThnC as shown by biochemical assays.

引用

页码：1985 / 1990

页数：6